A kind of detection method of xylo-oligosaccharide content
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A detection method and technology of xylo-oligosaccharide, applied in the field of analytical chemistry, to achieve the effects of system stability, good reproducibility and low consumption
Active Publication Date: 2017-08-29
BY HEALTH CO LTD
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Problems solved by technology
At present, there is no published detection method that can accurately detect the content of xylooligosaccharides
Method used
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Embodiment 1～3
[0051] The detection of embodiment 1~3 xylo-oligosaccharide content
[0052] Liquid chromatography conditions:
[0053] The chromatographic condition of table 1 embodiment 1~3 of the present invention
[0054]
[0055] 2. Preparation of reference substance stock solution:
[0056] Weigh 50 mg of xylose reference substance, add purified water to ultrasonically dissolve it, then dilute it to a 10 mL volumetric flask with purified water to prepare a standard stock solution with a concentration of 5.0 mg / mL, and store it at 4°C in the dark.
[0057] 3. Standard working curve drawing:
[0058] Take the above-mentioned standard stock solution 0.04mL, 0.1mL, 0.2mL, 0.4mL, 0.8mL into a 10mL volumetric flask, dilute to a concentration of 0.02mg / mL, 0.05mg / mL, 0.10mg / mL, 0.20mg / mL, 0.40mg / mL standard test solution, concentration from low to high were injected into the test and draw the working curve.
[0059] 4. Sample preparation:
[0060] Accurately weigh 650±50mg of the sampl...
Embodiment 1
[0086] Table 2 Example 1 xylo-oligosaccharide content detection result
[0087]
[0088] Note: The theoretical value of xylooligosaccharides in Qingchang powder is 8.10%.
Embodiment 4
[0089] Embodiment 4 linear range confirmation
[0090] Obtain the experimental data of standard product according to embodiment 1 detection method, see table 3:
[0091] Table 3 standard test data
[0092]
[0093] With the concentration C as the ordinate and the peak area A as the abscissa, draw a standard working curve, such as Figure 4 shown.
[0094] Linear evaluation: correlation coefficient R 2 It is 0.999, so this method is used to indirectly measure the content of xylo-oligosaccharides, showing good linearity between the concentration of 0.0378mg / mL and 0.4536mg / mL, which is in line with GB / T27404-2008 "Laboratory Quality Control Standards for Food Physical and Chemical Testing "Requirements [GB / T 27404-2008 requires correlation coefficient R 2 ≥0.99].
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Abstract
The invention relates to the field of analytical chemistry, in particular to a xylo-oligosaccharide content detection method. The detection method comprises steps as follows: a xylo-oligosaccharide test sample is taken and hydrolyzed, a hydrolyzed xylo-oligosaccharide test sample is obtained, HPLC (high performance liquid chromatography) is adopted for detection, the chromatographic condition of the HPLC is as follows: a saccharide column or an amino column is adopted, purified water or an acetonitrile aqueous solution is taken as a mobile phase for isocratic elution, and the xylo-oligosaccharide content is obtained according to an HPLC detection result. With the adoption of the detection method, interference of other functional saccharide cellulose can be eliminated, and the xylo-oligosaccharide content can be accurately detected; the xylo-oligosaccharide content detection method meets the requirement of the GB / T 27404-2008 Criterion on Quality Control of Laboratories-Chemical Testing of Food through linearity and precision tests and recovery rate tests, it proves that the detection method is scientific and effective, and the purpose of quality control on the xylo-oligosaccharide content detection can be achieved.
Description
technical field [0001] The invention relates to the field of analytical chemistry, in particular to a detection method for xylooligosaccharide content. Background technique [0002] Constipation is a common clinical symptom rather than a disease. It mainly refers to decreased frequency of defecation, decreased amount of feces, dry feces, and laborious defecation. There are many causes of constipation symptoms. Constipation has a prevalence of up to 27% in the population and can affect people of all ages. Women are more than men, and the elderly are more than young and middle-aged. Due to the high incidence of constipation and complicated etiology, patients often have many distresses, and when constipation is severe, it will affect the quality of life. [0003] As a new functional food ingredient, xylo-oligosaccharide is widely used in various functional foods due to its excellent processing characteristics and various physiological regulation functions. Xylo-oligosacchar...
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