TAK-438 liposome and preparation method and application thereof
A TAK-438, liposome technology, applied in the directions of liposome delivery, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as inability to use TAK-438 tablets, and achieve good antacid effect, Ease of operation, high solubility and bioavailability
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Embodiment 1
[0038] The present embodiment provides a TAK-438 liposome, comprising the following raw materials in parts by mass: lecithin 60mg, cholesterol 10mg, film-forming solvent 10mL, TAK-4381mg, buffer 40mL, the film-forming solvent is chloroform, the The buffer solution is a phosphate buffered saline solution, and the pH value of the phosphate buffered saline solution is 7.4.
[0039] Specifically include the following steps:
[0040] (1) Add 60mg lecithin and 10mg cholesterol into the reactor;
[0041] (2) Add 10mL of film-forming solvent and methanol solution containing TAK-4381mg to the above reactor to obtain a mixed solution, in which the mass ratio of TAK-438 to methanol is 1:1;
[0042] (3) Evaporate the solvent in the mixed solution to form a film, then add 40 mL of buffer solution, and rotate for 20 minutes with a rotary evaporator to obtain TAK-438 liposomes;
[0043] (4) Stir the TAK-438 liposomes for 3 hours, sonicate for 3 minutes; then filter through a 0.8um filter m...
Embodiment 2
[0055] This embodiment provides a TAK-438 liposome, the preparation method of which is similar to that of Example 1, the only difference being that the mass parts of raw materials are as follows: 25 parts of soybean lecithin, 5 parts of cholesterol, and 8 parts of film-forming solvent , 0.5 parts of TAK-438, 20 parts of buffer.
Embodiment 3
[0057] This embodiment provides a TAK-438 liposome, the preparation method of which is similar to that of Example 1, the only difference being that the mass parts of raw materials are as follows: 80 parts of soybean lecithin, 25 parts of cholesterol, and 258 parts of film-forming solvent , 2.5 parts of TAK-438, 60 parts of buffer.
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Abstract
Description
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