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A kind of sea cucumber alkaloid and its preparation method and application

An alkaloid and sea cucumber technology, which is applied in the directions of drug combination, organic chemistry, anti-tumor drugs, etc., can solve the problem that the anti-tumor activity of sea cucumber alkaloid compounds has not been reported, and achieve the effect of simple preparation method and low cost.

Inactive Publication Date: 2017-10-20
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the antitumor activity of sea cucumber alkaloids

Method used

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  • A kind of sea cucumber alkaloid and its preparation method and application
  • A kind of sea cucumber alkaloid and its preparation method and application
  • A kind of sea cucumber alkaloid and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) After taking out the viscera of 100kg of fresh sea cucumbers, put them in boiling water and cook for 20-30 minutes, and 100kg of sea cucumbers will produce about 50kg of sea cucumber boiling liquid;

[0033] (2) Get 20L of sea cucumber boiled liquid, adjust the pH value to 10, and centrifuge at 8000r / min for 40min to obtain the supernatant, and pass the obtained supernatant in batches through AB-8 type macroporous resin columns (5L each time), the flow rate 2BV / h, after the adsorption is saturated, rinse with 2L of deionized water, and then rinse with 2L of 20% ethanol solution;

[0034] (3) Elute the above-mentioned macroporous resin column with 6L of 70% ethanol solution, collect the eluent, concentrate under reduced pressure to extractum, and obtain sea cucumber extract;

[0035] (4) Redissolve the above-mentioned sea cucumber extract with 500mL of deionized water, centrifuge at 8000r / min for 40min to take the supernatant, extract and degrease the supernatant wit...

Embodiment 2

[0045] The in vitro antitumor test was carried out on the 6-OH erythracine prepared in Example 1, which showed that it had a moderately strong antitumor effect. The cell lines used in the test are internationally accepted tumor cell lines, namely: lung cancer cell A-549, NCI-H1299, liver cancer cell HepG2, prostate cancer cell PC-3 and breast cancer cell MCF-7.

[0046] 6-OH erythrine was formulated with DMSO at a standard concentration of 50 μg / mL, filtered through a 0.45 μL microporous membrane, and diluted sequentially to 25, 12.5, 6.25 and 3.125 μg / mL before use and added to the cells to be tested. The determination of antitumor activity adopts MTT method:

[0047]a) Collect tumor cells in the logarithmic growth phase at 1×10 5 / mL concentration inoculated in 96-well plate, 100 μl / well, at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours to make the cells adhere to the wall;

[0048] b) Aspirate the supernatant, add the maintenance solution containing the sample so...

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Abstract

The present invention discloses a sea cucumber alkaloid and a preparation method and application thereof. The sea cucumber alkaloid is 6-OH erysodine with a molecular formula C14H18N2O3, and a structural formula as follows. The sea cucumber alkaloid prepared by the present invention has significant inhibition effect on lung cancer cell A-549, NCI-H1299, prostate cancer cells PC-3 and breast cancer cells, but has weak inhibition effect on HepG2 cells. The invention provides basis for the development of novel anticancer drugs and has significant value on the development of marine drugs.

Description

technical field [0001] The invention specifically relates to a sea cucumber alkaloid and its preparation method and application. Background technique [0002] There are extremely rich resources and energy in the ocean, and the species in my country's sea area are also very diverse. With the continuous and rapid development of the marine industry, more and more entrepreneurs have set their sights on the ocean market, so nearly ten Over the past few years, my country's marine pharmaceutical industry has developed very rapidly, and domestic research on sea cucumbers in the phylum Echinodermata is also a hot field. [0003] Sea cucumbers are creatures of the class Holothunoider of the phylum Echinodermata. There are more than 1,200 species of sea cucumbers in the world, and there are more than 140 species in my country's sea areas. According to the shape of the tentacles, the presence or absence of tube feet and breathing trees, it can be divided into 6 orders: Dendrochirotida, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20A61P35/00
CPCC07D209/20
Inventor 林毅林淑芳
Owner HUAQIAO UNIVERSITY