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Method for cultivating photosynthetic microorganisms and method for producing oil

A technology for photosynthetic microorganisms and culture solution, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high cost of cultivating photosynthetic microorganisms

Active Publication Date: 2018-02-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defect of high cost of existing cultured photosynthetic microorganisms, to provide a method for cultivating photosynthetic microorganisms that can ensure the normal growth of photosynthetic microorganisms and reduce energy consumption, and is suitable for large-scale production

Method used

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  • Method for cultivating photosynthetic microorganisms and method for producing oil
  • Method for cultivating photosynthetic microorganisms and method for producing oil
  • Method for cultivating photosynthetic microorganisms and method for producing oil

Examples

Experimental program
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Effect test

Embodiment 1

[0048] This example is used to illustrate the method for cultivating photosynthetic microorganisms of the present invention.

[0049] Chlorella (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences) was cultivated in a device for cultivating photosynthetic microorganisms. Determination of Chlorella I 0 =30000lux. Use BG11 medium, nitrogen fertilizer is 0.3g / L urea, culture solution thickness is 5cm, the temperature of the culture solution is controlled by temperature adjustment zone 2 to 25-35°C, and the initial concentration of algae is OD 680 It is 0.25, and 2% (v / v) CO is introduced 2 Aerated culture, the amount of feed makes the pH value of the algae solution in the range of 6-10 during the culture process. During the cultivation process, artificial light source and sunlight are used for mixed cultivation, the light-dark cycle is 12:12, the light intensity is controlled to 10000lux during the day (12h), the paddle wheel 5 is driven to make the flow ...

Embodiment 2

[0051] This example is used to illustrate the method for cultivating photosynthetic microorganisms of the present invention.

[0052] Scenedesmus (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences) was cultivated in a device for cultivating photosynthetic microorganisms. Determination of Scenedesmus I 0 =30000lux. BG11 medium is used, nitrogen fertilizer is 0.3g / L urea, culture solution thickness is 8cm, the temperature of culture solution is controlled by temperature adjustment zone 2 to 25-35°C, and the initial concentration of algae is OD 680 It is 0.25, and 2% (v / v) CO is introduced 2 Aerated culture, the amount of feed makes the pH value of the algae solution in the range of 6-10 during the culture process. During the cultivation process, artificial light source and sunlight are used for mixed cultivation, the light-dark cycle is 12:12, the light intensity is controlled to 20000lux during the day (12h), the paddle wheel 5 is driven to make the f...

Embodiment 3

[0054] This example is used to illustrate the method for cultivating photosynthetic microorganisms of the present invention.

[0055] Monopodella (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences) was cultivated in a device for cultivating photosynthetic microorganisms. Determination of I 0 =30000lux. Use BG11 medium, nitrogen fertilizer is 0.3g / L urea, culture solution thickness is 10cm, the temperature of the culture solution is controlled by temperature adjustment zone 2 to 25-35°C, and the initial concentration of algae is OD 680 It is 0.25, and 2% (v / v) CO is introduced 2 Aerated culture, the amount of feed makes the pH value of the algae solution in the range of 6-10 during the culture process. During the cultivation process, artificial light source and sunlight are used for mixed cultivation, the light-dark cycle is 12:12, the light intensity is controlled to 40000lux during the day (12h), the paddle wheel 5 is driven to make the flow rate of...

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Abstract

The invention discloses a method for cultivating photosynthetic microorganisms. The method includes cultivating photosynthetic microorganisms in a device for cultivating photosynthetic microorganisms under conditions for cultivating photosynthetic microorganisms. The conditions for cultivating photosynthetic microorganisms include: under light, The flow of photosynthetic microorganisms is driven, and the flow rate of photosynthetic microorganisms increases with the increase of light intensity. Also disclosed is a method for producing oil, which includes microalgae cultivation, microalgae harvesting, separation and extraction of microalgae oil, and microalgae cultivation adopts the method for cultivating photosynthetic microorganisms as described above. The method of the invention can not only ensure the normal growth of photosynthetic microorganisms but also reduce energy consumption, and is suitable for large-scale production of photosynthetic microorganisms.

Description

technical field [0001] The present invention relates to a method for cultivating photosynthetic microorganisms and a method for producing oil. Background technique [0002] Photosynthetic microorganisms mainly include microalgae and photosynthetic bacteria. Since many useful products can be extracted from photosynthetic microorganisms, such as oils, proteins and pigments, their large-scale, high-efficiency, and low-cost cultivation has become the focus of attention. [0003] Microalgae are a class of low-level plants that grow in water, are diverse and widely distributed, and are cell factories driven by sunlight. Microalgal cells absorb CO through efficient photosynthesis 2 , convert light energy into chemical energy, store it in carbohydrates such as fat or starch, and release O 2 . The use of microalgae to produce bioenergy and chemicals can simultaneously achieve "replacing fossil energy and reducing CO 2 The advantages of "microalgae biotechnology" lie in the follow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C12P7/64C12R1/89
Inventor 朱俊英荣峻峰黄绪耕周旭华程琳宗保宁纪洪波
Owner CHINA PETROLEUM & CHEM CORP