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Rice seed specific expression promoter and application thereof

A gene expression and DNA molecule technology, applied in the field of rice seed-specific expression promoter, can solve the problems of aggravating plant burden, waste of resources, affecting plant morphological growth and development, etc., and achieves the effect of broad application and market prospect.

Inactive Publication Date: 2015-11-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

At present, constitutive promoters are widely used in plant genetic engineering, but some problems have gradually been exposed in the application. For example, it enables the expression of foreign genes in the whole plant, produces a large amount of foreign proteins or metabolites, and breaks the plant. The original metabolic balance increases the burden on the plant, affects the shape and normal growth and development of the plant, and even leads to the death of the plant, resulting in unnecessary waste of resources

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  • Rice seed specific expression promoter and application thereof
  • Rice seed specific expression promoter and application thereof
  • Rice seed specific expression promoter and application thereof

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Embodiment

[0029] 1. Experimental methods and steps

[0030] 1. Screening of rice seed-specific expression genes

[0031] By searching the RNA-seq data in the rice gene expression database in the "Rice Genome Annotation Project (RGAP)" website (http: / / rice.plantbiology.msu.edu / ), the tissue-specific expression genes were screened as candidate seeds specifically expressed genes. The RGAP website, funded by the National Foundation of the United States, provides the genome sequence and annotation information of 12 chromosomes of the rice subspecies "Nipponbare". Candidate promoter numbers, candidate gene numbers, RNA-seq data, and codes are shown in Table 1. According to the rice genome database information, about 2kb upstream of the gene start codon ATG was selected as the promoter and 5'UTR region of the gene. The LOC_Os02g25640 gene is endosperm-specific expression (JExpBot, 2008, 59(9):2417-2424).

[0032] Table 1 RNA-seq results of candidate genes in the database

[0033]

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Abstract

The invention discloses a rice seed specific expression promoter and application thereof. The sequence of the promoter is as shown in any one of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3, or the promoter has DNA molecule which is hybridized with the DNA sequence as shown in any one of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3 and has the functions of the promoter. The three promoters screened by the invention can be used for promoting the specific expression of target genes (such as cinH gene) in rice seeds, so that expression products of the target genes are accumulated in a specific special region, and the constitutive expression of the target genes in all tissues of rice is prevented to avoid influence on the growth of plants. The novel promoter disclosed by the invention can be extensive in application and market prospect in agricultural field.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rice seed-specific expression promoter and application thereof. Background technique [0002] A promoter is a DNA sequence located in the upstream region of a gene, which participates in the expression regulation of downstream genes and determines the transcription initiation, transcription efficiency and spatiotemporal specificity of the gene. Promoters can be divided into three categories according to their mode of action and function: constitutive promoters, inducible promoters, and tissue-specific promoters. At present, constitutive promoters are widely used in plant genetic engineering, but some problems have gradually been exposed in the application. For example, it enables the expression of foreign genes in the whole plant, produces a large amount of foreign proteins or metabolites, and breaks the plant. The original metabolic balance increases the burden on the...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/82C12N15/11C12N1/21C12N5/10A01H5/00
Inventor 韩志国区永祥
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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