Rice seed specific expression promoter and application thereof
A gene expression and DNA molecule technology, applied in the field of rice seed-specific expression promoter, can solve the problems of aggravating plant burden, waste of resources, affecting plant morphological growth and development, etc., and achieves the effect of broad application and market prospect.
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[0029] 1. Experimental methods and steps
[0030] 1. Screening of rice seed-specific expression genes
[0031] By searching the RNA-seq data in the rice gene expression database in the "Rice Genome Annotation Project (RGAP)" website (http: / / rice.plantbiology.msu.edu / ), the tissue-specific expression genes were screened as candidate seeds specifically expressed genes. The RGAP website, funded by the National Foundation of the United States, provides the genome sequence and annotation information of 12 chromosomes of the rice subspecies "Nipponbare". Candidate promoter numbers, candidate gene numbers, RNA-seq data, and codes are shown in Table 1. According to the rice genome database information, about 2kb upstream of the gene start codon ATG was selected as the promoter and 5'UTR region of the gene. The LOC_Os02g25640 gene is endosperm-specific expression (JExpBot, 2008, 59(9):2417-2424).
[0032] Table 1 RNA-seq results of candidate genes in the database
[0033]
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