A kind of silage and its preparation method and application
A technology of silage and inoculation loop, applied in the field of aquaculture, can solve the problems of poor quality, low utilization rate, waste, etc., and achieve the effects of reducing losses, promoting the generation of fermentation metabolites, and improving quality
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[0030] 1. Preparation of spare bacterial solution
[0031] (1) Take the slant of Pediococcus lactis, pick 2 rings of lawn with an inoculation loop, inoculate it in MRS medium, and activate it at 37°C for 22 hours to obtain the activated bacterial liquid; take the slant of Lactobacillus plantarum, and pick it with an inoculation loop 2 rings of lawn, inoculated in MRS medium, activated at 37°C for 24 hours to obtain activated bacterial liquid; take the slant of yeast, pick 2 rings of lawn with an inoculation loop, inoculate in wort medium, 30°C Activated for 24 hours under certain conditions to obtain activated bacterial liquid;
[0032] (2) Take the activated bacteria liquid of Pediococcus lactis, inoculate it in the MRS medium according to 2% inoculum amount, and cultivate it for 20 hours at 37° C. culture medium at 37°C for 22 hours to obtain a culture medium; take the activated yeast liquid, inoculate it into wort medium at a 2% inoculum size, and cultivate at 30°C for 20 ...
Embodiment 1
[0044] The alfalfa grown to the initial flowering stage was cut, dried in the sun until the water content reached 65%, and cut into 3cm pieces. Inoculate Pediococcus lactis in MRS liquid medium, and inoculate and activate it at 37°C for 22 hours; inoculate Lactobacillus plantarum in MRS liquid medium, and inoculate it at 37°C for 26 hours; inoculate yeast in wort Activation culture in medium at 28°C for 24 hours. Inoculate the activated Pediococcus lactis in the MRS liquid medium at an inoculation amount of 2%, and culture it statically at 37°C for 20 hours; inoculate the activated Lactobacillus plantarum in the MRS liquid medium at an inoculum amount of 2%, Cultivate statically at 37°C for 24 hours; inoculate the activated yeast in the wort medium according to the inoculum amount of 2%, and cultivate on a shaker at 28°C for 20 hours. Mix Pediococcus lactis culture solution, Lactobacillus plantarum culture solution, and yeast culture solution in a ratio of 5:4:1 to form a com...
Embodiment 2
[0046] The alfalfa growing to the initial flowering stage was cut, dried in the sun until the water content reached 60%, and cut into 2cm pieces. Inoculate Pediococcus lactis in MRS liquid medium, and inoculate and activate it at 37°C for 22 hours; inoculate Lactobacillus plantarum in MRS liquid medium, and inoculate it at 37°C for 26 hours; inoculate yeast in wort Activation culture in medium at 28°C for 24 hours. The activated Pediococcus lactis was inoculated in the MRS liquid medium at an inoculum size of 4%, and cultured at 37° C. for 20 hours; Cultivate statically at 37°C for 24 hours; inoculate the activated yeast in the wort medium according to the inoculum amount of 4%, and cultivate on a shaker at 28°C for 20 hours. Mix Pediococcus lactis culture solution, Lactobacillus plantarum culture solution, and yeast culture solution in a ratio of 5:4:1 to form a composite bacterial solution; use 5% bran by weight of silage to absorb the composite bacterial solution of 0.3% b...
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