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Method for preparing colon cancer resistance notopterygium extract powder in scale mode

A technology of extract powder and colon cancer, which is applied in the field of large-scale preparation of anti-colon cancer notopterygium extract powder, can solve the problems that there are no literature reports on anti-colon cancer extract powder, and achieve stable, controllable and repeatable quality Good, cheap solvent effect

Inactive Publication Date: 2015-12-02
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the Chinese patent (102367256A) reports that notopterygium isoimperatorin extract is obtained by two steps of supercritical carbon dioxide extraction and crystallization, but there is no literature report on the large-scale preparation of anti-colon cancer extract powder from notopterygium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 A large-scale preparation method of anti-colon cancer notopterygium extract powder, comprising the following steps:

[0027] (1) Extraction: After crushing 5.0Kg of notopterygium medicinal materials to 60 mesh, add ethanol solution or methanol solution with a volume fraction of 95% for alcohol extraction, and obtain the filtrate after filtration; Dry under reduced pressure to a paste, and obtain 984.6 g of notopterygium alcoholic extract.

[0028] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:10L, the temperature of 95°C, the extraction times of 3 times, and each extraction time of 1h.

[0029] (2) Liquid-liquid distribution and enrichment: the ethanol extract of notopterygium was dispersed with analytically pure water 30 times its mass to obtain a dispersion; the dispersion was extracted 3 times with 30 times its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; The ethyl acetate extract was dr...

Embodiment 2

[0035] Example 2 A large-scale preparation method of anti-colon cancer notopterygium extract powder, comprising the following steps:

[0036] (1) Extraction: After crushing 10.0Kg of notopterygium medicinal materials to 120 mesh, add ethanol solution or methanol solution with a volume fraction of 95% for alcohol extraction, and obtain the filtrate after filtration; Drying under reduced pressure until it was paste-like, 1921.9 g of notopterygium alcoholic extract was obtained.

[0037] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:20L, the temperature of 60°C, the number of extractions once, and the extraction time of each time is 3h.

[0038] (2) Liquid-liquid distribution and enrichment: the ethanol extract of notopterygium was dispersed with analytically pure water 50 times its mass to obtain a dispersion; the dispersion was extracted 3 times with 50 times its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; ...

Embodiment 3

[0044] Example 3 A large-scale preparation method of anti-colon cancer notopterygium extract powder, comprising the following steps:

[0045] (1) Extraction: After crushing 20.0Kg of notopterygium to 80 mesh, add ethanol solution or methanol solution with a volume fraction of 95% for alcohol extraction, and obtain the filtrate after filtration; Dry under reduced pressure to a paste, and obtain 3829.6 g of notopterygium alcoholic extract.

[0046] Among them: the alcohol extraction conditions refer to the solid-liquid ratio of 1kg:15L, the temperature of 80°C, the extraction times of 2 times, and the extraction time of each extraction is 2h.

[0047] (2) Liquid-liquid distribution and enrichment: the ethanol extract of notopterygium was dispersed with analytically pure water 40 times its mass to obtain a dispersion; the dispersion was extracted 3 times with 40 times its volume of ethyl acetate, and combined to obtain an ethyl acetate extract; The ethyl acetate extract was dr...

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Abstract

The invention relates to a method for preparing colon cancer resistance notopterygium extract powder in a scale mode, which comprises steps: 1 exaction: notopterygium medical materials are extracted by alcohol and filtered after being smashed, and is decompress and dried to be a paste state, thereby obtaining notopterygium alcohol extract; 2 liquid-liquid distribution enrichment: the notopterygium alcohol extract is smashed, extracted, decompressed and dried to obtain a notopterygium ethyl acetate position; 3 resin column chromatography edulcoration: the notopterygium ethyl acetate position is separated through a resin column after being dissolved, is eluted in a gradient mode, 90% methanol contents are collected, the 90% methanol contents are decompressed and dried to obtain notopterygium extract coarse powder; 4 silica gel column chromatography separation: the notopterygium extract coarse powder is separated and eluted through a silica gel column chromatography after being dissolved, and is detected through a thin-layer chromatography, and fractions which are 0.1-0.8 in radio frequency (Rf) values are collected, id decompressed and dried to obtain yellow powder-shaped notopterygium extract powder. The method for preparing the colon cancer resistance notopterygium extract powder in the scale mode is simple in process, easy to carry out in a scale mode and is steady and controllable in quality.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a large-scale preparation method of notopterygium extract powder for anti-colon cancer. Background technique [0002] Notopterygium ( Notopterygium incisum TingexH.TChang) is the dried root and branch root of the plant of the genus Notopterygium in the family Umbelliferae, mainly distributed in Sichuan, Yunnan, Qinghai and Gansu. Modern studies have shown that the chemical components in Notopterygium mainly include monoterpenes, sesquiterpenes, phenylpropanoids, coumarins, amino acids, organic acids and organic acid esters, among which coumarins are known for their significant anti- Tumor activity is well known. [0003] At present, the Chinese patent (102367256A) reports that notopterygium isoimperatorin extract is obtained by two steps of supercritical carbon dioxide extraction and crystallization. However, there is no literature report on the lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/237A23L1/29A61P35/00A23L33/00
Inventor 张莉党军王启兰梅丽娟邵赟陶燕铎岳会兰
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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