Nandina domestica fire power primary culture medium

Abstract

The invention discloses a medium formula which is most suitable for nandina domestica fire power primary culture. The medium formula comprises modified MS+zeatin (0.6mg / L)+indole acetic acid (0.2mg / L)+glucose (15g / L)+agar powder (6g / L). The hormone combination in the medium of the invention is reasonable, primary culture and rooting are combined in one step, stem sections can take roots in a primary culture process, the culture process is simplified, and the culture time is shortened. Meanwhile, a bud number of primary culture plants can be improved, the plants grow robustly, which is conductive to next step transgenerational propagation, the medium can provide more high quality seedlings for the nandina domestica fire power, amd is conducive to widespread promotion, and greater economic benefits are created.

Description

technical field

[0001] The invention belongs to the technical field of tissue culture, in particular to the rapid propagation technology of flame nandina tissue culture. Background technique

[0002] Flame nandina is a new type of greening seedlings. Its leaves are bright and bright like fire in winter, and are very popular among the people. Because the flame nandina plants are short, the stem nodes are short, the internodes are only 1 / 10 of ordinary nandina, and the branches are thin and weak, so cutting propagation is difficult and the propagation speed is slow. Since the Shanghai Botanical Garden introduced this species as a collection of resources in 1995, the research on flame nandina in my country has only begun. The cutting method is usually used in production, which has a long reproduction cycle and extremely low reproduction rate, and cannot achieve good results in production. With the continuous development of tissue culture technology in recent years, especially ...

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