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A kind of medium for primary culture of flame nandina

A kind of technology of flame nandina and first-generation cultivation, applied in tissue culture, microorganism, horticulture, etc., can solve the problems of low proliferation coefficient and slow cultivation speed, and achieve the effect of simplifying the cultivation process, increasing the number of buds, and growing robustly.

Active Publication Date: 2017-07-11
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the relevant literature on the tissue culture of flame nandina that has been published so far, the proliferation coefficient of tissue culture rapid propagation is low and the culture speed is slow, so it is particularly important to carry out the primary culture of flame nandina

Method used

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  • A kind of medium for primary culture of flame nandina
  • A kind of medium for primary culture of flame nandina
  • A kind of medium for primary culture of flame nandina

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Embodiment Construction

[0021] Medium formula: improved MS+zeatin (0.6mg / L)+indole acetic acid (0.2mg / L)+glucose (15g / L)+agar powder (6g / L).

[0022] The formula for the improved MS is:

[0023] Macroelements: (NH 4 ) 2 SO 4 600mg / L, CO(NH 2 )500mg / L, KNO 3 950mg / L, CaCl 2 2H 2 O220mg / L, MgSO 4 ·7H 2 O 185mg / L, KH 2 PO 4 85mg / L.

[0024] Trace elements: KI 0.41mg / L, H 3 BO 3 3.1mg / L, MnSO 4 4H 2 o 2 1.15mg / L, ZnSO 4 ·7H 2 O4.3mg / L, Na 2 MoO 4 2H 2 O 0.125mg / L, iron salt: FeSO 4 ·7H 2 O(27.8), Na 2 -EDTA·2H 2 O(37.3).

[0025] Organic substances: inositol 50mg / L, niacin 0.5mg / L, pyridoxine hydrochloride (vitamin B6) 0.5mg / L, thiamine hydrochloride (vitamin B1) 0.1mg / L, calcium pantothenate (vitamin B5) 1mg / L L, vitamin C 2mg / L, glycine 2.0mg / L.

[0026] Add each material according to the amount required by the above medium formula, and boil to fully mix and dissolve all the materials. Adjust the pH of the culture medium to 5.7, pack in required containers and sterilize...

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Abstract

The invention discloses a medium formula most suitable for the primary cultivation of flame nandina: improved MS+zeatin (0.6mg / L)+indole acetic acid (0.2mg / L)+glucose (15g / L)+agar powder (6g / L). The ratio of hormones in the culture medium of the present invention is reasonable, the primary culture and the rooting culture are combined into one step, and the stem segment can take root during the primary culture, which simplifies the culture process and shortens the culture time. At the same time, the number of buds of the first-generation cultured plants can be increased, and the plants grow robustly, which is conducive to the subsequent expansion of the next generation. Provide more high-quality seedlings for flame nandina, which is conducive to large-scale promotion and creates greater economic benefits.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, in particular to the rapid propagation technology of flame nandina tissue culture. Background technique [0002] Flame nandina is a new type of greening seedlings. Its leaves are bright and bright like fire in winter, and are very popular among the people. Because the flame nandina plants are short, the stem nodes are short, the internodes are only 1 / 10 of ordinary nandina, and the branches are thin and weak, so cutting propagation is difficult and the propagation speed is slow. Since the Shanghai Botanical Garden introduced this species as a collection of resources in 1995, the research on flame nandina in my country has only begun. The cutting method is usually used in production, which has a long reproduction cycle and extremely low reproduction rate, and cannot achieve good results in production. With the continuous development of tissue culture technology in recent years, especially ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
Inventor 贾思振张立伟王媛花颜志明
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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