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Method for catalyzing and degrading polygalacturonic acid in paper making white water via aspergillus niger whole cells

A polygalacturonic acid and whole-cell catalyst technology, applied in the field of whole-cell catalysis, can solve the problems of difficult separation and extraction of products, complex metabolites, low substrate conversion rate, etc., and achieves improved catalytic efficiency, high catalytic efficiency, and enzyme good stability

Inactive Publication Date: 2015-12-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the fermentation method, whole-cell catalysis overcomes the shortcomings of the fermentation method, such as long production cycle, complex metabolites, low substrate conversion rate, difficult product separation and extraction, and high energy consumption.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Add 1.0g bran, 0.3g commercial pectin (pectin, fromcitrusfruits) and (NH4) to 100mL distilled water 2 SO 4 Solid 2.0g, K 2 HPO 4 Solid 0.1g, KCl solid 0.05g, MgSO 4 ·7H 2 O0.05g, Na 2 SO 4 0.05g, FeSO 4 ·7H 2 O0.0001g, adjust the pH to 6.0 to form an expansion medium, insert 4ml concentration of 1 × 10 8 / L Aspergillus niger (Aspergillus niger GIM3.462 was purchased from the Institute of Microbiology, Chinese Academy of Sciences) spore suspension, cultured at 30°C, 180r / min on a shaker for 5 days, collected Aspergillus niger by centrifugation, and freeze-dried for 24 hours to make a whole-cell catalyst; , 5-dinitrosalicylic acid colorimetric method (DNS method) was used to measure and compare the pectinase activity produced by Aspergillus niger and the commercial alkaline pectinase activity; add 5g / 5ml of L pectin substrate; preheated in a water bath at 50°C for 5min, then added 5mL of phosphate buffer solution with a pH of 8 respectively; Glue enzyme, shake ...

Embodiment 2

[0029] In 100mL distilled water, add 2.0g bran, 0.2g commercial pectin (pectin, fromcitrusfruits), (NH 4 ) 2 SO 4 Solid 2.0g, K 2 HPO 4 Solid 0.1g, KCl solid 0.05g, MgSO 4 ·7H 2 O0.05g, Na 2 SO 4 0.05g, FeSO 4·7H 2 O0.0001g, adjust the pH to 6.0 to form an expansion medium, insert 4ml concentration of 1 × 10 8 / L Aspergillus niger (Aspergillus niger GIM3.462 was purchased from the Institute of Microbiology, Chinese Academy of Sciences) spore suspension, cultured at 30°C, 170r / min on a shaker for 5 days, collected Aspergillus niger by centrifugation, and freeze-dried for 24 hours to make a whole-cell catalyst; , 5-dinitrosalicylic acid colorimetric method (DNS method) was used to measure and compare the pectinase activity produced by Aspergillus niger and the commercial alkaline pectinase activity; add 5g / 5ml of L pectin substrate; preheated in a water bath at 50°C for 5min, then added 5mL of phosphate buffer solution with a pH of 8 respectively; Glue enzyme, shake ...

Embodiment 3

[0031] Add 1.0g bran, 0.3g commercial pectin (pectin, fromcitrusfruits), (NH 4 ) 2 SO 4 Solid 2.5g, K 2 HPO 4 Solid 0.1g, KCl solid 0.05g, MgSO 4. ·7H 2 O0.05g, Na 2 SO 4 0.05g, FeSO 4 ·7H 2 O0.0001g, adjust the pH to 6.0 to form an expansion medium, insert 4ml concentration of 1 × 10 8 / L Aspergillus niger (Aspergillus niger GIM3.462 purchased from the Institute of Microbiology, Chinese Academy of Sciences) spore suspension, cultured at 35°C, 200r / min on a shaker for 6 days, collected Aspergillus niger by centrifugation, and freeze-dried for 24 hours to make a whole-cell catalyst; , 5-dinitrosalicylic acid colorimetric method (DNS method) was used to measure and compare the pectinase activity produced by Aspergillus niger and the commercial alkaline pectinase activity; add 5g / 5ml of L pectin substrate; preheated in a water bath at 50°C for 5min, then added 5mL of phosphate buffer solution with a pH of 8 respectively; Glue enzyme, shake it up immediately, and put i...

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Abstract

The invention discloses a method for catalyzing and degrading polygalacturonic acid in paper making white water via aspergillus niger whole cells. In the method, dormant aspergillus niger is activated on a bevel activation medium; fermentation is conducted in an expanded medium to achieve the aspergillus niger; the polygalacturonic acid works as a model object for pectin substance in the paper making white water; after the polygalacturonic acid solution with certain concentration is prepared, PGA is catalyzed and degraded via the polygalacturonic acid whole cells; and a reaction condition is optimized. Whole cells biological catalysis is utilized to process paper making white water to overcome deficiencies of low reaction efficiency of a chemical floculence method, a low selectivity, environment pollution and imbalanced white water system charge; and the method for catalyzing and degrading polygalacturonic acid in paper making white water via the aspergillus niger whole cells is advantaged by temperate reaction conditions, simple and controllable reaction process, high catalyzing efficiency and environment protection.

Description

technical field [0001] The invention relates to the technical field of whole cell catalysis, in particular to a method for biocatalytically degrading polygalacturonic acid in papermaking white water by using Aspergillus niger whole cells and optimization of application conditions. Background technique [0002] Paper mills have always been a major player in water and sewage discharge. Considering the environment and production costs, the white water system of paper mills is required to be closed and circulated to reduce the use of clean water and waste water discharge. However, with the improvement of the degree of closed circulation of white water and the increase of the number of white water cycles, harmful substances in white water are gradually accumulated, which affects the efficiency of the paper machine and the quality of paper. Studies have shown that harmful substances in white water are mainly dissolved and colloidal substances (DCS for short). These substances hav...

Claims

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Application Information

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IPC IPC(8): D21F1/66
Inventor 赵光磊稂雄妃眭梁梁李晓凤
Owner SOUTH CHINA UNIV OF TECH
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