Preparation method for paraffin section of ectropis obliqua male adult internal genitalia and tissue picking and moving tool for application of preparation method
A technique for internal genitalia and paraffin sectioning, which is applied in the field of insect anatomy and physiology, to achieve the effects of shortening the tissue fixation time, improving the sectioning effect and microscopic observation quality, and improving the production efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0056] 1) Material collection: In physiological saline, dissect male adults between 10 and 11 hours after eclosion, remove the internal genitalia, and take out the testes. The entire sampling time is controlled within 1 minute to ensure the freshness of the materials.
[0057] 2) Fixation: Using a tissue picking tool, move each testis tissue into a fixative solution for 5 hours.
[0058] 3) Washing and dehydration: Because the fixative is yellow, the test material needs to be washed 3 times with 70% alcohol, and the cleaning time is controlled at 20 minutes each time to remove the yellow color of the fixative. Then wash 3 times with 80% → 95% → pure alcohol, each time 15min.
[0059] 4) Transparency: The tissue material is subjected to pure alcohol + TO-type biological transparent solvent equal mixture solution → TO-type biological transparent solvent, each for 12 minutes.
[0060] 5) Wax immersion: in a wax melter, the temperature is set at 60°C, and the material is passed t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com