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A method for preparing a paraffin section of the internal genitalia of a tea geometrid adult male and a tissue picking tool using the method

A technique for internal genitalia and paraffin sectioning, which is applied in the field of insect anatomy and physiology to achieve the effect of improving production efficiency, improving sectioning effect and microscopic observation quality, and reducing human harm.

Inactive Publication Date: 2017-12-22
TEA RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unique structure of the internal genitalia of Lepidoptera such as Tea Geometrid, it is obviously not advisable to completely copy the traditional tissue section method

Method used

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  • A method for preparing a paraffin section of the internal genitalia of a tea geometrid adult male and a tissue picking tool using the method
  • A method for preparing a paraffin section of the internal genitalia of a tea geometrid adult male and a tissue picking tool using the method
  • A method for preparing a paraffin section of the internal genitalia of a tea geometrid adult male and a tissue picking tool using the method

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Experimental program
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Effect test

Embodiment 1

[0056] 1) Material collection: In physiological saline, dissect male adults between 10 and 11 hours after eclosion, remove the internal genitalia, and take out the testes. The entire sampling time is controlled within 1 minute to ensure the freshness of the materials.

[0057] 2) Fixation: Using a tissue picking tool, move each testis tissue into a fixative solution for 5 hours.

[0058] 3) Washing and dehydration: Because the fixative is yellow, the test material needs to be washed 3 times with 70% alcohol, and the cleaning time is controlled at 20 minutes each time to remove the yellow color of the fixative. Then wash 3 times with 80% → 95% → pure alcohol, each time 15min.

[0059] 4) Transparency: The tissue material is subjected to pure alcohol + TO-type biological transparent solvent equal mixture solution → TO-type biological transparent solvent, each for 12 minutes.

[0060] 5) Wax immersion: in a wax melter, the temperature is set at 60°C, and the material is passed t...

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Abstract

The invention belongs to the technical field of insect anatomy and physiology, and in particular relates to a method for preparing a paraffin section of the internal genitalia of a tea geometrid male adult and a tissue picking tool using the method. In the fixation step of the preparation method, the main components of the fixative by volume percentage are: 50 parts of saturated picric acid, 25 parts of formaldehyde, 5 parts of glacial acetic acid, and 10 parts of 70% alcohol; 8h. The tissue slices made by this method have remarkable characteristics such as better slice effect and clearer tissue structure under microscopic observation. The tissue picking tool includes a support body with a mosquito-repellent coil structure and a handle extending outward from the support support body; The gap between adjacent rings constitutes a leak for the solution to leak down. The tool enables reliable movement of tissue during different treatments and effectively ensures tissue integrity.

Description

technical field [0001] The invention belongs to the technical field of insect anatomy and physiology, and in particular relates to a method for preparing a paraffin section of the internal genitalia of a tea geometrid male adult and a tissue picking tool using the method. Background technique [0002] Tea geometrid is one of the main tea plant pests in my country, and its harmfulness is widespread in all tea regions in China, among which the damage is most serious in Jiangnan tea region. The insect has 6-7 generations per year, and the alternation of generations is serious. Adults lay a lot of eggs, and indoor measurements show that under the conditions of suitable temperature and humidity and sufficient food, the average number of eggs laid by a single female reaches more than 400. The maximum food consumption period of the larvae is the 3rd to 5th instar stage, and when the damage is serious, it will form a broom branch, and it will take 2 to 3 years to restore the origin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 张家侠孙钦玉赵强夏先江
Owner TEA RES INST ANHUI ACAD OF AGRI SCI
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