A method for preparing a paraffin section of the internal genitalia of a tea geometrid adult male and a tissue picking tool using the method
A technique for internal genitalia and paraffin sectioning, which is applied in the field of insect anatomy and physiology to achieve the effect of improving production efficiency, improving sectioning effect and microscopic observation quality, and reducing human harm.
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[0056] 1) Material collection: In physiological saline, dissect male adults between 10 and 11 hours after eclosion, remove the internal genitalia, and take out the testes. The entire sampling time is controlled within 1 minute to ensure the freshness of the materials.
[0057] 2) Fixation: Using a tissue picking tool, move each testis tissue into a fixative solution for 5 hours.
[0058] 3) Washing and dehydration: Because the fixative is yellow, the test material needs to be washed 3 times with 70% alcohol, and the cleaning time is controlled at 20 minutes each time to remove the yellow color of the fixative. Then wash 3 times with 80% → 95% → pure alcohol, each time 15min.
[0059] 4) Transparency: The tissue material is subjected to pure alcohol + TO-type biological transparent solvent equal mixture solution → TO-type biological transparent solvent, each for 12 minutes.
[0060] 5) Wax immersion: in a wax melter, the temperature is set at 60°C, and the material is passed t...
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