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A kind of mercury chelated immune complex and its preparation method and application
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An immune complex, chelating technology, applied in the medical field, can solve problems such as functional changes, elevations, and changes in circulating immune complexes
Inactive Publication Date: 2017-03-01
广东腾湃医疗股份有限公司
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But how does environmental pollution stimulate the body to significantly increase the CIC content? Is it caused by one or multiple pollutants? Whether the increase in CIC will cause damage to the body and increase the susceptibility of the body to diseases? all to be studied
As we all know, circulating immune complexes are proteins of special significance, and mercury can act on proteins in multiple systems throughout the body. Whether mercury can act on circulating immune complexes will change the content, activity and function of circulating immune complexes , there is still a lack of relevant research on these
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preparation example Construction
[0062] Among them, the first preparation method includes
[0063] A) the step of synthesizing mercury chelated immune complex;
[0064] B) The step of purifying the mercury chelating immune complex: using immunoaffinity chromatography to remove the carrier protein, specific antibody and mercury ions that do not participate in the reaction in the mercury chelating immune complex synthesized in step A).
[0065] Specifically, in the preparation method of the mercury chelated immune complex,
[0066] (1) preparing chelating agent solution: chelating agent is dissolved, and is mixed with chelating agent solution;
[0068] (Ⅲ) Stir overnight: add the chelating agent solution in step (I) to the carrier protein solution in step (II), stir and act on a shaker for 2-30 hours to obtain a mixed solution;
[0069] (Ⅳ) Dialysis bag pretreatment: add EDTA-NaH...
Embodiment 1
[0164] In this example, ITCBE was purchased from Japan Tongren Chemical Research Institute, item number M030;
[0165] The following borate buffer solution, its preparation method example: Weigh 0.31g boric acid and dissolve it in 400mLddH 2 In O, use 0.1Mol / L NaOH aqueous solution to adjust the pH to 9.0, and set the volume to 500mL, to obtain;
[0166] The following EDTA-NaHCO 3 Solution preparation method example: Take 1.86g EDTA·2H 2 O and 16.8 g NaHCO 3 , dissolved in 900mLddH 2 In O, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000mL, autoclave, and store at 25±2°C;
[0167] The following dialysis bags had a molecular weight cut-off of 14000 and were purchased from Bioshop Inc; the dialysis bags were processed as follows: Place the dialysis bag in 500 mL volume of EDTA-NaHCO 3 solution, boil for 10min; discard EDTA-NaHCO 3 solution with ddH 2 Rinse with O, then boil with 500mL 5mmol / L EDTA for 10min; 2 O cleaning, adding a large amount of ddH 2 O Soak the ...
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Abstract
The invention discloses a mercury-chelated immune complex, a preparation method and an application thereof. The mercury-chelated immune complex is one from the following complexes: 1) a complex formed by combining mercury ion to an immune complex; 2) a complex formed by combining mercury ion to a carrier protein with an antibody capable of being specially combined with the carrier protein; or 3) a complex formed by combining mercury ion to an immune globulin and then combining the immune globulin with the carrier protein. The mercury-chelated immune complex has a relative specificity and is used for detecting whether human serum in people in a certain region contains mercury or not and detecting the content of mercury, thereby indirectly reflecting mercury pollution degree of this region.
Description
technical field [0001] The invention belongs to the field of medicine, and in particular relates to a mercury chelated immune complex and a preparation method and application thereof. Background technique [0002] Immune complexes (IC) are the product of antigen-antibody binding. According to the definition of the National Committee for Scientific and Technical Terms, IC refers to the complex formed by the combination of antibody and soluble antigen. It is divided into two categories, one is the fixed immune complexes in the tissue, and the other is the immune complexes that are free in the blood and become circulating immune complexes (circulating immune complexes, CIC). Immune complexes play an indispensable role in clearing and destroying various antigens, and the result of the body's immune response is the continuous production of immune complexes. When the number of antigens (or antibodies) in the body is slightly more than that of antibodies (or antigens), an immune c...
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