Rose fermentation puree as well as preparation method and application thereof
A technology of fermenting puree and rose, which is applied in the biological field, can solve the problems of environmental damage, achieve the effects of simplifying production steps, maintaining natural activity, and avoiding the loss of active ingredients
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Embodiment 1
[0034] Embodiment 1, a kind of rose fermentation protoplasm and preparation method thereof:
[0035] 1. Activation of strains:
[0036] A ring of colonies of Saccharomyces pastorianus was picked and placed in a liquid medium, and placed in a shaker to activate the strains to obtain an activated Saccharomyces pastorianus liquid.
[0037] 2. Purification of strains:
[0038] The activated bacterial solution obtained in step 1 was serially diluted and plated so as to obtain a single colony to obtain purified Saccharomyces pasturii.
[0039] 3. Expansion and cultivation of strains:
[0040] Inoculate the purified bacterial classification obtained in step 2 into the YPD medium (pH value is 7.0), cultivate in a shaker at 25°C, and when the OD value=0.5-1.0, obtain the fermented bacterial liquid (the bacterial classification In the logarithmic phase, the concentration is 10 5 -10 8 CFU / ml).
[0041] 4. Obtaining rose fermented puree:
[0042] Inoculate 15ml of the fermented ba...
Embodiment 2
[0043] Embodiment 2, the application of rose fermented protoplasm as cosmetics:
[0044] 1. Properties of rose fermented puree cosmetics:
[0045] The appearance of the rose fermented puree prepared in Example 1 is viscous liquid, and the color is light yellow to brownish yellow. The pH value is 5.2-6.8, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 for cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented extract meets the quality requirements of cosmetics.
[0046] The composition analysis of the rose fermented puree is carried out. The protein detection method refers to GB5009.5-2010; the crude polysaccharide detection method refers to GB / T5009.8-2008; the flavonoid detection method refers to GB / T5009.124-2003; GB / T8313-2008, the obtained results are as follows:
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