A high camellia fermented puree cosmetic and its preparation method and application
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A technology for fermenting puree and high mountain tea, applied in the biological field, can solve problems such as damage to the environment, and achieve the effects of simplifying production steps, saving production costs, and ensuring stability
Active Publication Date: 2019-01-08
SHANGHAI BIOTRULY BIOTECH CO LTD
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Problems solved by technology
However, this method introduces organic matter in the extraction process, which not only damages the environment, but also has potential safety hazards in the application process.
Method used
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Embodiment 1
[0034] Embodiment 1, a kind of preparation method of high camellia fermented protoplasm
[0035] 1. Activation of strains
[0036] Pick a ring of Saccharomyces cerevisiae colonies in a liquid medium, put them into a shaker to activate the strains, and obtain the activated Saccharomyces cerevisiae liquid.
[0037] 2. Purification of strains
[0038] The activated bacterial solution obtained in step 1 was serially diluted and plated to obtain a single colony to obtain purified Saccharomyces cerevisiae.
[0039] 3. Expansion and cultivation of strains
[0040] Inoculate the purified bacterial classification obtained in step 2 into the YPD medium (pH value is 7.0), cultivate it in a shaker at 25°C, and when the OD value=0.4-0.8, obtain the fermented bacterial liquid (the bacterial classification In the logarithmic phase, the concentration is 10 4 -10 7 CFU / ml).
[0041] 4. Obtaining the fermented puree of high mountain tea
[0042] Inoculate 7mL of the fermented bacteria li...
Embodiment 2
[0043] Embodiment 2, the application of high camellia fermented puree cosmetics:
[0044] 1. Properties of high mountain tea fermented puree cosmetics:
[0045] The appearance of the high camellia fermented puree cosmetic (supernatant) prepared in this example is viscous liquid, and the color is light yellow to brownish yellow. The pH value is 5.2-6.3, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 for cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented extract meets the quality requirements of cosmetics.
[0046] The component analysis of this high camellia fermented puree cosmetic is carried out. The protein detection method refers to GB5009.5-2010; the crude polysaccharide detection method refers to GB / T 5009.8-2008; the flavonoid detection method refers to GB / T 5009.1...
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Abstract
The invention discloses a high mountain tea fermentation raw stock cosmetic and a preparation method thereof. High mountain tea fermentation raw stock is prepared in the following steps that high mountain tea dry powder, water and bacterial liquid are mixed to obtain an initial system, fermented cultivation is conducted on the initial system, and accordingly the high mountain tea fermentation raw stock cosmetic is obtained. Fermentation bacteria are saccharomyces cerevisiae. The application of the high mountain tea fermentation raw stock cosmetic to preparation of a product with the skin whitening and / or anti-ageing functions also belongs to the protective range of the invention. The product is the cosmetic and can specifically be any one of masks or essence or toner. The high mountain tea fermentation raw stock cosmetic prepared through the method does not contain chemical components and can be directly used as finished products of the masks or the essence or the toner for use. Compared with other products on an existing market, the cosmetic is more natural, and no negative effect on the skin can be caused; moreover, active ingredients with the small molecular weight can be obtained, and can be fully absorbed by the skin more easily.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high camellia fermented puree cosmetic and its preparation method and application. Background technique [0002] High mountain tea refers to the tea produced between 1000 meters and 2600 meters above sea level. It is a kind of semi-fermented tea and belongs to oolong tea. The appearance is spherical or hemispherical. High mountain tea contains more tea polyphenols, caffeine and other substances than ordinary tea, which has the functions of regulating fat metabolism, anti-cancer, anti-radiation, anti-bacterial, anti-aging skin, and reducing the damage of ultraviolet radiation in the sun to the skin. Refreshing, diuretic and other effects. [0003] Ultrasonic method is most commonly used for the extraction of active substances in the existing high mountain tea: after the dry product of high mountain tea is crushed, add ethanol of a certain concentration, sonicate for a ...
Claims
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