Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of rabbit anti ovotransferrin allergen polyclone antibody and immunoassay method thereof

A technology of ovotransferrin and polyclonal antibody, which is applied in the field of macromolecular protein immunity, can solve the problems of less allergic research and allergic effects, and achieve the effects of good inhibition rate, good sensitivity and precision, and high titer

Inactive Publication Date: 2015-12-23
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ovotransferrin (OVTorGald3) is one of the main allergens of egg allergens, and its content accounts for about 12% of the total egg white protein. It is a glycoprotein bound to iron ions. Ovotransferrin is free Ovotransferrin usually has antibacterial activity in its form. Ovotransferrin is composed of two domains, namely N domain and C domain. It is a single peptide chain consisting of 686 amino acids. It can combine with iron ions and is a This kind of glycoprotein contains 15 disulfide bonds in each protein molecule, and has antioxidant properties. At present, there are relatively few researches on the allergy to ovotransferrin. Ovotransferrin is more sensitive to temperature changes, and high temperature can make it Allergies are severely affected

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of rabbit anti ovotransferrin allergen polyclone antibody and immunoassay method thereof
  • Preparation method of rabbit anti ovotransferrin allergen polyclone antibody and immunoassay method thereof
  • Preparation method of rabbit anti ovotransferrin allergen polyclone antibody and immunoassay method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]The immunized animals are male white rabbits from New Zealand, about 3 months old, weighing about 1.5 kg, and the immunogen is ovotransferrin.

[0046] The method of immunization was subcutaneous and intramuscular injection. Four booster immunizations were carried out after the initial immunization. The booster immunizations were 2 weeks, 4 weeks, 6 weeks and 8 weeks after the initial immunization. Whole blood was collected from the femoral artery one week after the fifth immunization. The method is:

[0047] (1) Initial immunization: The immunogen and complete Freund's adjuvant were mixed in a volume of 1:1, and fully emulsified through a well-sealed glass syringe until the immunogen and adjuvant were completely mixed and became milky white. The immunization dose was 1mg / animal for animal immunization;

[0048] (2) Booster immunization: Immunogen and Freund's incomplete adjuvant are mixed and emulsified in equal volumes to immunize animals; the amount of immunization i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of rabbit anti ovotrasferrin allergen polyclone antibody and an immunoassay method thereof. The preparation method comprises the steps that ovotransferrin rabbits are directly immune to New Zealand white rabbits, after immunization is conducted for five times, whole blood is taken from femoral arteries, and ovotransferrin antibody serum is obtained; the antibody peridium amount is optimized, the dilution ratio of the antibody, confining liquid and the PH value of a sample buffer solution are detected, and an indirect competitive ELISA method among the ovotransferrin is established. The antibody prepared through the method is high in potency and good in inhibition rate, and the established method is good in sensitivity and accuracy.

Description

technical field [0001] The invention belongs to the technical field of macromolecular protein immunization; in particular, it relates to the preparation of ovotransferrin to immunize animals, specific antibodies and the establishment of immune analysis methods. Background technique [0002] Ovotransferrin (OVTorGald3) is one of the main allergens of egg allergens, and its content accounts for about 12% of the total egg white protein. It is a glycoprotein bound to iron ions. Ovotransferrin is free Ovotransferrin usually has antibacterial activity in its form. Ovotransferrin is composed of two domains, namely N domain and C domain. It is a single peptide chain consisting of 686 amino acids. It can combine with iron ions and is a This kind of glycoprotein contains 15 disulfide bonds in each protein molecule, and has antioxidant properties. At present, there are relatively few researches on the allergy to ovotransferrin. Ovotransferrin is more sensitive to temperature changes, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/531G01N33/68G01N21/31
CPCG01N33/531G01N21/31G01N33/6803
Inventor 王硕生威李晶刘冰陆旸
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com