Culturing method of polyploid anoectochilus roxburghii variety

A cultivation method and technology of clematis, applied in the field of plant cultivation, can solve problems such as insufficient benefit for growers, low multiplication rate, small clematis, etc., so as to improve the induction rate, increase the permeability, Excellent effect

Active Publication Date: 2015-12-30
GUANGXI XIANGCHENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a long time to adopt seed direct seeding, root division propagation, cutting propagation, etc., and

Method used

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  • Culturing method of polyploid anoectochilus roxburghii variety

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The breeding method of polyploid golden clematis kind, comprises the following steps:

[0039] (a), culture the seeds of diploid Aurea clematis on the first medium for 27 days to obtain protocorms, wherein the first medium is: MS medium containing BA1.2mg / L, KT1.2mg / L L, NAA0.15mg / L, sucrose 35g / L, agar 5g / L, banana 17g / L;

[0040] (b), culture the protocorm in dark for 3 days, then soak it in 5 μmol / L asulphin solution for 36 hours;

[0041] (c), the soaked protocorm is transferred to the first medium to continue culturing to obtain clustered buds;

[0042] (d), transfer to the rooting medium and cultivate in the rooting medium for 38 days to obtain the complete tissue culture seedlings of roots, stems and leaves after the clustered shoots, wherein the rooting medium is: MS medium containing NAA0.8mg / L, gac 0.6g / L , Banana 25g / L;

[0043] (e), the polyploid plant obtained through chromosome detection and screening compared with the parental diploid, the plant is tal...

Embodiment 2

[0046] The breeding method of polyploid golden clematis kind, comprises the following steps:

[0047] (a), the seeds of diploid Aurea clematis were cultured on the first medium for 30 days to obtain protocorms, wherein the first medium was: MS medium containing BA1.0mg / L, KT1.0mg / L L, NAA0.1mg / L, sucrose 30g / L, agar 5g / L, banana 20g / L;

[0048] (b), culture the protocorm in dark for 4 days, then soak it in 10 μmol / L ansulphin solution for 20 hours;

[0049] (c), the soaked protocorm is transferred to the first medium to continue culturing to obtain clustered buds;

[0050] (d), transfer to the rooting medium and cultivate for 40 days in the rooting medium to obtain the complete tissue culture plantlets of roots, stems and leaves, wherein the rooting medium is: MS medium contains NAA0.5mg / L, gac 0.5g / L , Banana 20g / L;

[0051] (e), the polyploid plant obtained through chromosome detection and screening compared with the parental diploid, the plant is tall, the stem segment i...

Embodiment 3

[0054] The breeding method of polyploid golden clematis kind, comprises the following steps:

[0055] (a), culture the seeds of diploid Aurea clematis on the first medium for 32 days to obtain protocorms, wherein the first medium is: MS medium containing BA0.8mg / L, KT0.7mg / L L, NAA0.05mg / L, sucrose 20g / L, agar 4.5g / L, banana 25g / L;

[0056] (b), the protocorm was cultured in dark for 5 days, then soaked in 8 μmol / L ansulphin solution for 30 hours;

[0057] (c), the soaked protocorm is transferred to the first medium to continue culturing to obtain clustered buds;

[0058] (d), transfer to the rooting medium and cultivate for 45 days in the rooting medium to obtain the complete tissue culture plantlets of roots, stems and leaves, wherein the rooting medium is: MS medium contains NAA0.4mg / L, gac 0.4g / L , Banana 18g / L;

[0059] (e), the polyploid plant obtained through chromosome detection and screening compared with the parental diploid, the plant is tall, the stem segment is...

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Abstract

The invention relates to the field of plant culture and particularly relates to a culturing method of a polyploid anoectochilus roxburghii variety. The culturing method comprises the following steps of culturing a diploid anoectochilus roxburghii seed to obtain a protocorm; soaking the protocorm in 5-15mu mol/L of an oryzalin solution to treat for 10-36 hours; further culturing the soaked protocorm to obtain a clustered shoot; after dividing, transferring the clustered shoot to a rooting medium to culture to obtain a tissue culture seedling with complete root, stem and leaves; and sieving the tissue culture seedling to obtain a polyploid plant. According to the culturing method of the polyploid anoectochilus roxburghii variety, provided by the invention, the anoectochilus roxburghii protocorm with very strong dividing capacity is used as an inducing material, the protocorm is soaked for the specific time by using the oryzalin solution with the specific concentration, and then, a polyploid anoectochilus roxburghii plant can be obtained through screening in cultured plants, wherein the induction rate ranges from 43.2% to 44.8%, and the obtained polyploid anoectochilus roxburghii plant is favorable in character.

Description

technical field [0001] The invention relates to the field of plant cultivation, in particular to a method for cultivating polyploid golden clematis varieties. Background technique [0002] Golden clematis is the whole herb of the genus Anoectochilus in the family Orchidaceae, also known as golden clematis and golden silk grass. It is a traditional precious medicinal material widely used by the people. It has the functions of clearing away heat and detoxification, expelling wind and dampness, nourishing yin and moistening lungs, lowering blood pressure, and calming the liver. Folks are used to treat various diseases such as tuberculosis, lung-heat cough, rheumatoid arthritis, convulsions in children, bruises, snake bites, etc., and have good curative effects. It has a small plant type, beautiful leaf shape, and golden-yellow network arrangement of leaf veins, which has high ornamental value. In recent years, the use of clematis in the treatment of difficult diseases such as...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 苏钰琴莫燕兰盘飞兰杨杰花韦祥意罗文正张华英
Owner GUANGXI XIANGCHENG BIOLOGICAL TECH
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