Method for detoxifying cottonseed cakes by using microorganism fermentation

A technology for microbial fermentation and cottonseed meal, applied in the field of cottonseed meal, can solve the problems of low cost, many by-products, increase protein content and quality, etc., and achieve the effects of low cost, simple production process, and increased protein content and quality.

Inactive Publication Date: 2016-01-06
雷春生
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: for the toxic substance free gossypol contained in the current cottonseed cake, the general use of chemical and physical dephenolization treatment methods has poor operability and high cost, and the protein quality in the cottonseed cake after detoxification is poor, and by-products Many, seriously affecting the problem of the application of cottonseed cake, a method for detoxifying cottonseed cake by microbial fermentation is provided. The invention first uses cottonseed cake to carry out high-pressure sterilization, and then mixes it with fresh pothos juice, filters, Mix the filter residue and peptone and other nutrients to make a medium, culture Aspergillus niger and yeast strains respectively, and then ferment after cultivation, filter the filter residue after fermentation, wash the filter residue with acetone and water respectively, and then dry it. The invention uses Aspergillus niger and yeast microorganisms for cultivation and fermentation, so that free gossypol can be combined with protein amino acids to form combined gossypol, which not only achieves the purpose of detoxification, but also increases the protein content and quality of cottonseed cake without producing by-products , and the production process is simple and the cost is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] First, take 500g of cottonseed cake, put it into a pulverizer and grind it, sieve and screen out 50-mesh particles, add water to the cottonseed cake powder at a solid-liquid mass ratio of 1:1 for mixing, stir well and put it into high pressure In a sterilizing pot, cook at 100°C and cook at 20°C, control the pressure at 0.2Mpa, filter after cooking, and obtain a solid filter residue; take 500g of fresh green radish leaves, first chop them, and then squeeze the juice to obtain fresh Green radish juice, add the above-mentioned cottonseed cake filter residue and 3g ferrous sulfate heptahydrate to the green radish juice, carry out ultrasonic dispersion, ultrasonic 10min, to obtain a uniform suspension; take 150mL of the above suspension, 10g peptone, 9g beef extract , mixed with 15g of glucose, stirred evenly, added 2L of distilled water and 3g of triammonium citrate to it, continued to stir and mix, adjusted the pH of the suspension to 6 with oxalic acid solution with a mas...

example 2

[0026]First, take 550g of cottonseed cake, put it into a pulverizer and grind it, sieve and screen out 55 mesh particles, add water to the cottonseed cake powder at a solid-to-liquid mass ratio of 1:1 for mixing, stir well and put it into high pressure In a sterilizing pot, cook at 108°C and cook at 25°C, control the pressure at 0.3Mpa, filter after cooking, and obtain a solid filter residue; take 550g of fresh green radish leaves, first chop them, and then squeeze them to obtain fresh Green radish juice, add the above-mentioned cottonseed cake filter residue and 4g ferrous sulfate heptahydrate to the green radish juice, carry out ultrasonic dispersion, ultrasonic 13min, obtain a uniform suspension; take 170mL of the above suspension, 13g peptone, 10g beef extract , mixed with 17g of glucose, stirred evenly, added 2.1L of distilled water and 4g of triammonium citrate to it, continued to stir and mix, adjusted the pH of the suspension to 6.5 with oxalic acid solution with a mass...

example 3

[0029] First, take 600g of cottonseed cake, put it into a pulverizer and grind it, sieve and screen out 60-mesh particles, add water to the cottonseed cake powder at a solid-liquid mass ratio of 1:1 for mixing, stir well and put it into high pressure In a sterilizing pot, cook at 115°C and cook at 30°C, control the pressure at 0.4Mpa, filter after cooking, and obtain a solid filter residue; take 600g of fresh green radish leaves, first chop them, and then squeeze the juice to obtain fresh Green radish juice, add the above-mentioned cottonseed cake filter residue and 5g ferrous sulfate heptahydrate to the green radish juice, carry out ultrasonic dispersion, ultrasonic 15min, obtain a uniform suspension; take 200mL of the above suspension, 15g peptone, 12g beef extract , mixed with 20g of glucose, stirred evenly, added 2.2L of distilled water and 5g of triammonium citrate to it, continued to stir and mix, adjusted the pH of the suspension to 7 with oxalic acid solution with a mas...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detoxifying cottonseed cakes by using microorganism fermentation and belongs to the field of cottonseed cakes. The method comprises the following steps: carrying out high-temperature sterilization on the cottonseed cakes, and then mixing the cottonseed cakes with fresh scindapsus aureus juice and filtering; mixing filtering dreg with nutriments including peptone and the like to prepare a culture medium; culturing aspergillus niger strains and saccharomycetes strains respectively; fermenting after culturing; after fermenting, filtering to obtain filtering dreg; and washing the filtering dreg with acetone and water respectively, and drying to obtain the product. According to the method disclosed by the invention, microorganisms including aspergillus niger and saccharomycetes are cultured and fermented so that free gossypol is combined with protein amino acids to form combined gossypol, and furthermore, the aim of detoxifying is realized and the content and quality of protein in the cottonseed cakes are improved; and byproducts are not produced, a production process is simple and the cost is low.

Description

technical field [0001] The invention discloses a method for detoxifying cottonseed cake by microbial fermentation, which belongs to the field of cottonseed cake. Background technique [0002] my country is a major cotton producing country in the world, with an annual output of more than 8 million tons of cottonseed. Among them, cottonseed accounts for about 50%~55%, the protein content of cottonseed is 35~38%, and the crude protein content of cottonseed cake after oil extraction can reach 36~44%. However, because it contains free gossypol, which is toxic to livestock and poultry, the abundant protein resources have not been fully utilized. At present, the utilization rate of cottonseed meal feed in my country is less than 30%. [0003] There are many detoxification methods for cottonseed meal. Among them, the heat treatment method is to use high temperature and high pressure conditions to convert most of the free gossypol with strong toxic effects into non-toxic bound gos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
Inventor 雷春生王志慧
Owner 雷春生
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap