Application of surfactin to cosmetic

A technology of surfactin and cosmetic composition, applied in the application field of surfactin in cosmetics, can solve the problems of irregularity, drug effect destruction, liver burden and the like

Inactive Publication Date: 2016-01-06
陆振冈 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of oral administration is that the drug is absorbed slowly and irregularly in the body, which makes the drug unable to achieve therapeutic effect; moreover, the drug must first pass through the liver before reaching the blood, which destroys the drug effect and causes a burden to the liver; Drugs cannot be taken orally because they cannot be absorbed or are irritating in the intestines; the biggest disadvantage of oral administration is that the drugs will cause side effects in the body, causing patients to experience physical discomfort such as nausea and vomiti

Method used

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  • Application of surfactin to cosmetic
  • Application of surfactin to cosmetic
  • Application of surfactin to cosmetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Anti-aging test of embodiment 1 supersurin on human skin and mouse embryonic fibroblasts

[0067] Experimental materials and methods

[0068] 1. Experimental cell lines

[0069] Humanskinfibroblast (CCD-966SK) was purchased from BCRC Center for Biological Resources Conservation and Research—No.: 60153.

[0070] 2. MouseBALB / 3T3embryo (BALB / 3T3CloneA31) was purchased from BCRC Center for Biological Resources Conservation and Research - number: 60009.

[0071] 3. Experimental animals

[0072] Single-sex BALB / cByJNarl strain mice were purchased from the National Experimental Animal Center, with a body weight of about 200-250 kg. The feeding conditions were artificial light for 12 hours a day, humidity 75% and temperature 25°C, and were raised in an ocean with air-conditioning equipment. University life science animal room, and provided adequate feed and drinking water by special personnel.

[0073] 4. Anti-aging gene expression test

[0074] (1) Human and mouse gene c...

Embodiment 2

[0094] Example 2 Surfactin has anti-photoaging and anti-oxidation effects

[0095] 1. Surfactin has the function of anti-aging and repairing

[0096] After dividing the cells into plates, culture them for 4 hours and fix them on the petri dish, use the ultraviolet lamp tube of UV-A wavelength as the source of light stress, and irradiate the experimental cells in the ultraviolet hybridization box with an energy concentration of 0J / cm 2 、10J / cm 2 and 15J / cm 2 After irradiation, 25, 50, 75, 100 and 125 μM concentrations of surfin were added, and after 24 hours of culture, the cell viability was tested by MTT method.

[0097] Depend on image 3 It can be seen that surfin has the function of anti-photoaging and repairing; when the mouse embryonic fibroblasts were irradiated with 10J / cm 2 Surfactin at concentrations of 25, 50, 75, 100 and 125 μM all had the effect of increasing cell viability after exposure to ultraviolet light, and the concentration of surfin at 75 μM had the b...

Embodiment 3

[0101] Example 3 Surfactin Effects on the Growth of Collagen in Mouse Embryo Fibroblasts

[0102] 1. Collagen Concentration Analysis

[0103] Collagen concentration analysis kit (Sircol TM solublecollagenassaykit) to analyze the total concentration of collagen in the culture medium. The analysis method is briefly described as follows:

[0104] Take the sample to be analyzed and 0.1 mL of standard substance of different concentrations and put them in a 1.5 mL centrifuge tube. Add 1mL dyereagent, shake the shaker for 35min. Centrifuge at 12000rpm for 10min, pour off the supernatant. Carefully remove the residual liquid from the edge of the centrifuge tube with absorbent paper, do not touch the sediment. Add 1mL of Alkalireagent to dissolve the precipitate. After the color is stable, take out 0.2mL and place it in a 96-well plate, and use ELISAreader to read the absorbance at 570nm.

[0105] 2. Surfactin has the effect of increasing collagen in mouse embryonic fibroblasts ...

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Abstract

The invention provides a biosurfactant produced from microorganism Bacilus subtilis-surfactin. The surfactin is natural ring-type lipopeptid with an annular structure formed from seven amino acids; the surfactin has the functions of anti-aging, anti-wrinkle, skin-penetrating power for cosmetic (permeation-promoter), foaming agent, emulsifying agent, etc.

Description

technical field [0001] The present invention relates to the application method of surfactin, in particular to the application method of surfactin in cosmetics. Background technique [0002] 1. Human skin structure and its aging factors [0003] The skin is the main organ on the surface of the human body, and its thickness varies depending on the location, age and gender; and the main function of the skin is the first barrier of the human body, which can prevent the internal tissues of the human body from being directly damaged by external environmental factors, such as: ultraviolet radiation, temperature The skin is easily stimulated by external factors to activate the aging mechanism to metabolize damaged cells. [0004] (1) Cell aging and gene defect [0005] Aging begins from cells and gradually affects tissues and organs, causing the structure and function of various parts of tissues and organs to gradually decline. In Leonard Hayflick's research in 1963, he cultured ...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/34A61K8/73A61K8/67A61K8/92A61Q19/08
Inventor 陆振冈王心玫许烜瑞
Owner 陆振冈
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