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Human bone morphogenetic protein 2 mature peptide and expression of mature peptide

A protein and bone cell technology, which is applied to peptide/protein components, animal/human peptides, peptide sources, etc., can solve the problems that no one continues to study BMP2, etc., and achieve the effect of improving curative effect and increasing expression

Active Publication Date: 2016-01-06
珠海深泓鑫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the more truncated BMP2 has not been pursued by anyone other than the present inventors

Method used

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  • Human bone morphogenetic protein 2 mature peptide and expression of mature peptide
  • Human bone morphogenetic protein 2 mature peptide and expression of mature peptide
  • Human bone morphogenetic protein 2 mature peptide and expression of mature peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Expression of rhBMP2 of the present invention

[0021] The present inventors discovered rhBMP2 whose amino acid sequence is shown in SEQ ID NO: 2, and carefully designed its expression-optimized coding gene (nucleotide shown in SEQ ID NO: 1) to be expressed and prepared in E. coli. In short, Shanghai Jierui Biotechnology Co., Ltd. was commissioned to artificially synthesize the gene, and then ligated into the pEVT vector (NewEnglandBiolabs) between the EcoRI and HindIII restriction sites, and transformed into E. coli BL21 (DE3). Screened on 50 mg / L ampicillin LB plate, the expression vector contained in the positive bacteria was named pEVT-hBMP2, the plasmid was extracted, and the sequencing was correct.

[0022] The positive bacteria were inoculated into 5mL LB medium, cultured at 30°C and 200r / min for 12h, and then inoculated into 400mL LB medium at 2% (V / V) inoculum, and cultured at 30°C, 200r / min for 8h. Then fermentation is carried out, and the 400mL fermente...

Embodiment 2

[0025] Example 2 Experiment of promoting bone formation of rhBMP2 of the present invention

[0026] The rhBMP2 of the present invention and the BMP2 of Chinese Patent Application No. 201410310953 and Chinese Patent Application No. 200910045832 were used to conduct a bone differentiation calcification test. Specifically, take the bone marrow fluid of 1 SD rat (male) and add it to DMEM culture medium containing 200ml / L fetal bovine serum, 100U / ml penicillin and 100Lg / ml streptomycin at 37℃, 50ml / L Cell culture was carried out with carbon dioxide, and the culture medium was changed every 3 days until the bone marrow stromal cells were 80% confluent, digested with 2.5g / L trypsin, and subcultured at a ratio of 1:2. Take the bone marrow stromal cells passed down to the 4th generation to 10 4 The amount per well was inoculated into a 96-well plate, the culture medium was discarded after 24 hours, and then randomly divided into 4 groups (all groups on the same 96-well plate, a total of t...

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Abstract

The invention provides a human bone morphogenetic protein 2. The human bone morphogenetic protein 2 is mature peptide and has an amino acid sequence which is represented by SEQ ID NO:2 or is obtained by adding, deleting and / or substituting one or several amino acid residues on the sequence represented by SEQ ID NO:2. The invention further provides a pharmaceutical composition which contains the human bone morphogenetic protein 2 and is used for promoting differentiation and calcification of bones.

Description

Technical field [0001] The present invention belongs to the technical field of protein or polypeptide. Specifically, the present invention relates to human bone morphogenetic protein 2 (especially recombinantly produced, referred to as rhBMP2), its medicines, and applications for promoting osteogenic differentiation and calcification. Background technique [0002] Bone morphogenetic protein (BMP), also known as human bone morphogenetic protein, except for human bone morphogenetic protein 1 (BMP1), the rest belong to the transforming growth factor-β superfamily, among which human bone morphogenetic protein 2 (BMP2) has been studied more frequently For example, Chinese Patent Application No. 98113048 discloses soluble recombinant human bone morphogenetic protein 2 for the treatment of hematopoietic dysfunction and injury, in which BMP2 is a wild-type protein; Chinese patent application No. 200610032800 discloses a recombinant human bone morphogenetic protein- 2 expression method, i...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12C12N15/70C12N1/21A61K38/17A61P19/00
CPCA61K38/00C07K14/47C12N15/70
Inventor 筴文奎
Owner 珠海深泓鑫生物科技有限公司
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