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Polyacrylamide gel peptization instant solid composite and preparing method thereof

A technology of polyacrylamide gel and solid composition, which is applied in the field of polyacrylamide gel dispersion and instant solid composition and its preparation, which can solve the difficult powder and granule molding process and tablet preparation process, and the tedious adjustment of pH value , troublesome operation and other problems, to achieve the effect of superior electrophoretic buffer performance, saving preparation time, and convenient storage

Active Publication Date: 2016-01-13
广东易优生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, these liquid acids are strong acids, highly corrosive, and volatile in the air, requiring smoke chambers and other warning facilities, which are difficult to apply to powder, granule molding and tablet preparation processes, and Adjusting the pH value is a cumbersome and unstable process, and the operation is troublesome

Method used

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  • Polyacrylamide gel peptization instant solid composite and preparing method thereof
  • Polyacrylamide gel peptization instant solid composite and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] 5%T / 3.5%C laminated polyacrylamide gel dispersed instant solid composition prescription composition: 48.34g of acrylamide, 1.66g of methylenebisacrylamide, 15.15g of trishydroxymethylaminomethane, 0.87g of citric acid g, 1.0 g of sodium lauryl sulfate, 5 g of lactose, 8 g of mannitol, and 300.3 g of PVPK. The dosage form prepared from the raw materials of the polyacrylamide gel-dispersed instant solid composition can be powder, granule or tablet.

[0054] The preparation method of the polyacrylamide gel dispersed instant solid composition powder of 5%T / 3.3%C laminated glue in this embodiment is:

[0055] Step 1: In an environment with a relative humidity of 40% and a temperature of 22-30°C, crush and grind acrylamide, pass through a 100-mesh sieve, and store for future use. At the same time, methylenebisacrylamide, trishydroxymethylaminomethane, citric acid, sodium lauryl sulfate, lactose, mannitol, and PVPK30 were respectively pulverized and ground, passed through a 1...

Embodiment 2

[0071] 12%T / 3.3%C separating gel polyacrylamide gel dispersed instant solid composition prescription composition: acrylamide 116g, methylenebisacrylamide 4g, tris-hydroxymethylaminomethane 45.43g, citric acid 0.46g, ten Dialkyl sodium sulfate 1.0g, lactose 10g, mannitol 10g, PVPK 300.5g. The dosage form prepared from the raw materials of the polyacrylamide gel-dispersed instant solid composition can be powder, granule or tablet.

[0072] In this embodiment, the preparation method of the polyacrylamide gel dispersed instant solid composition powder of 12%T / 3.3%C separating gel is:

[0073] Step 1: In an environment with a relative humidity of 10% and a temperature of 4-30°C, crush and grind acrylamide, pass through a 200-sieve, and store for later use. At the same time, methylenebisacrylamide, trishydroxymethylaminomethane, citric acid, sodium lauryl sulfate, lactose, mannitol, and PVPK30 were respectively pulverized and ground, then passed through 200 sieves, and stored separ...

Embodiment 3

[0089] The prescription of the polyacrylamide gel dispersion instant solid composition of 5%T / 5%C laminated glue consists of: 47.5g of acrylamide, 2.5g of methylenebisacrylamide, 9.08g of trishydroxymethylaminomethane, and 11.26g of glycine g, 10.5 g of serine, 5.64 g of glycolic acid, 3 g of lactose, 5 g of mannitol, 0.1 g of dextrin, and 0.4 g of magnesium stearate.

[0090] In this embodiment, the preparation method of the polyacrylamide gel dispersed instant solid composition of 5%T / 5%C laminated glue is:

[0091] Step 1: In an environment with a relative humidity of 40% and a temperature of 22-30°C, crush and grind acrylamide, pass through a sieve of 100 mesh or more, and store it for later use. At the same time, methylenebisacrylamide, trishydroxymethylaminomethane, glycolic acid, sodium lauryl sulfate, lactose, mannitol, dextrin, and magnesium stearate were respectively pulverized and ground, and then passed through a 100-mesh sieve, respectively Save for later.

[00...

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Abstract

The invention relates to the technical field of polyacrylamide gel, in particular to a polyacrylamide gel peptization instant solid composite and a preparing method thereof. The composite comprises a monomer which is capable of achieving free radical polymerization and contains acrylamide, a monomer capable of achieving crosslinking, a gel making buffer agent, a pH regulator and a solid forming auxiliary material. The peptization instant solid composite is prepared by mixing the solid forming auxiliary material with a polyacrylamide gel making reagent. In use, polyacrylamide gel can be obtained simply by dissolving the polyacrylamide gel peptization instant solid composite in water as needed, operation is easy and convenient, polyacrylamide gel preparing time is saved greatly, property and performance are stable, storage is convenient, and retention cycle is long; meanwhile, tablets are easy to prepare, dosage is accurate, and bulk production can be conducted to enable the polyacrylamide gel to be more stable and reliable, batch difference to be smaller, repeatability to be higher and cost to be lower.

Description

technical field [0001] The invention relates to the technical field of polyacrylamide gel, in particular to a polyacrylamide gel dispersed instant solid composition and a preparation method thereof. Background technique [0002] In the field of research and diagnosis, polyacrylamide gel is a widely used separation medium for separating biological macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA), polypeptides, proteins and their complexes. The basic device for using this technique is a thin tube open at both ends or a sandwich of two flat plates. Thin tubes and flat plates are made of materials with certain support strength, such as glass and plastics. First, add the unsolidified polyacrylamide solution into the mold of the above shape. After the polyacrylamide is solidified into a gel, the biological sample can be added to one end of the gel, and the positive and negative electrodes are respectively added to the two ends of the gel. Mobilize biolo...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K9/16A61K9/20A61K47/32C08F220/56C08F222/38
CPCA61K9/14A61K9/16A61K9/20A61K47/32C08F220/56C08F222/38
Inventor 郑杉水陈一凡杨艳逄淑召郭正科王隽
Owner 广东易优生物医药有限公司
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