Application and pharmaceutical composition of total glucosides of paeony in preparing medicine for preventing or treating nerve cell damage caused by microwave radiation
A technology of nerve cell damage and total glucosides of paeony, which is applied in the field of medicine, can solve the problems that research and development still need to be strengthened, and achieve the effect of reducing nerve cell damage, repairing nerve cells, and reducing the content
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Embodiment 1
[0058] 1. Culture induced PC12 cells
[0059]Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / Centrifuge at 1000 rpm for 10 minutes, discard the supernatant, add 4 ml of the above-mentioned medium, pipette evenly, and suck into the culture bottle , change the medium every other day, 37 degrees Celsius, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF and 1% fetal bovine serum.
[0060] Observing the cells in culture through a cell inverted microscope, the inventors found that before NGF induction, PC12 cells were round, with strong refraction, and semi-adherent growth. Afte...
Embodiment 2
[0079] Example 2 Effect of total glucosides of paeony on changes in ROS content in PC12 cells induced by microwave radiation
[0080] 1. Culture and induction of PC12 cells
[0081] Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / Centrifuge at 1000 rpm for 10 minutes, discard the supernatant, add 4 ml of the above-mentioned medium, pipette evenly, and suck into the culture bottle , change the medium every other day, 37 degrees Celsius, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF and 1% fetal bovine serum.
[0082] Observing the cells in culture through a cell inverte...
Embodiment 3
[0093] Example 3 Effect of total glucosides of paeony on changes in MDA content in PC12 cells induced by microwave radiation
[0094] 1. Culture and induction of PC12 cells
[0095] Take out the PC12 cell line with the characteristics of neurons and neurosecretory cells from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of 10% fetal bovine serum, 5% horse serum, and 100 units of penicillin / Centrifuge at 1000 rpm for 10 minutes, discard the supernatant, add 4 ml of the above-mentioned medium, pipette evenly, and suck into the culture bottle , change the medium every other day, 37 degrees Celsius, 5% CO 2 Cultivation and passage under conditions; after the above-mentioned cultured cell line was passed for 3 generations, it was induced to differentiate into neuron-like cells by using DMEM medium with 5 ng / ml NGF and 1% fetal bovine serum.
[0096] Observing the cells in culture through a cell inverted ...
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