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Application of astragalosides to preparation of medicine for preventing or treating microwave radiation induced myocardial cell injury, pharmaceutical composition and food

A technology for myocardial cell damage and total astragalus glycosides, which is applied in the field of medicine and can solve problems such as research and development that needs to be strengthened.

Inactive Publication Date: 2016-01-13
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the research and development of drugs that can be used to prevent and treat heart damage caused by microwave radiation still needs to be strengthened.

Method used

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  • Application of astragalosides to preparation of medicine for preventing or treating microwave radiation induced myocardial cell injury, pharmaceutical composition and food
  • Application of astragalosides to preparation of medicine for preventing or treating microwave radiation induced myocardial cell injury, pharmaceutical composition and food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Effect of total astragalosides on changes in ROS content in H9c2 cardiomyocytes induced by microwave radiation

[0058] 1. Culture H9c2 cardiomyocytes

[0059]Take out the H9c2 cardiomyocyte cell line from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, then add 1.5 ml of DMEM containing 10% fetal bovine serum, 100 units / ml of penicillin and 100 μg / ml of streptomycin for culture culture medium (dubecco'sminimallessentialmedium, DMEM), centrifuged at 1000 rpm for 10 minutes, discarded the supernatant, added 4 ml of the above-mentioned medium and pipetted it evenly, then sucked it into a culture bottle, and changed the medium every other day, at 37 degrees Celsius, 5% CO 2 Cultivate and subculture under the same conditions; the above-mentioned cultured cell lines are passed for 3 generations and then used for later use.

[0060] 2. Microwave irradiation of H9c2 cardiomyocytes

[0061] The H9c2 cardiomyocy...

Embodiment 2

[0070] Example 2 Effect of total astragalosides on changes in LDH content in H9c2 cardiomyocytes induced by microwave radiation

[0071] 1. Culture H9c2 cardiomyocytes

[0072] Take out the H9c2 cardiomyocyte cell line from liquid nitrogen, put it into water at 37 degrees Celsius, suck it into a centrifuge tube after melting, and then add 1.5 ml of Dubecco minimum containing 10% fetal bovine serum, penicillin 100 units / ml and streptomycin 100 μg / ml Essential medium (dubecco'sminimallessentialmedium, DMEM) culture medium, centrifuged at 1000 rpm for 10 minutes, discarded the supernatant, added 4 ml of the above-mentioned medium and pipetted it evenly, then sucked it into the culture bottle, changed the medium every other day, 37 degrees Celsius, 5 %CO 2 Cultivate and subculture under the same conditions; the above-mentioned cultured cell lines are passed for 3 generations and then used for later use.

[0073] 2. Microwave irradiation of H9c2 cardiomyocytes

[0074] The H9c2 ...

Embodiment 3

[0086] Example 3 Effect of total astragalosides on myocardial cell damage in rats induced by microwave radiation

[0087] Ten secondary male Wistar rats were randomly divided into 2 groups, 5 rats in the distilled water control group and 5 rats in the astragaloside group. Use the microwave radiation source of the Academy of Military Medical Sciences to uniformly irradiate the whole body, and the average power density of the microwave radiation source is 30mW / cm 2 , irradiated for 15 minutes. Rats in each group were intragastrically administered on the day after radiation, once a day, for 14 consecutive days, the dose of total astragalosides was 40 mg / kg / d, and the solvent of the injection was distilled water. The injection volume is 0, that is, only the distilled water is injected.

[0088] On the 14th day after radiation, that is, the 1st day after drug withdrawal, the rats were killed by dislocation, and the heart tissue of the rat was taken, fixed in 10% buffered formalin...

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Abstract

The invention discloses application of astragalosides to preparation of a medicine for preventing or treating microwave radiation induced myocardial cell injury, a pharmaceutical composition and food. According to the application of astragalosides to preparation of the medicine, the medicine is used for preventing or treating microwave radiation induced myocardial cell injury. Therefore, astragalosides can be used for preparing the medicine for preventing and treating microwave radiation induced myocardial cell injury, further, the prepared medicine can be used for administration prevention for people or animals probably suffering from microwave radiation, or can be used for administration treatment for people or animals suffering from microwave radiation induced myocardial cell injury so as to achieve the purposes of reducing myocardial cell injury and repairing injured myocardial cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the use of total astragalosides in the preparation of medicines for preventing or treating myocardial cell damage caused by microwave radiation, a pharmaceutical composition and food. Background technique [0002] The biological hazard of microwave radiation has attracted more and more attention from scholars at home and abroad. The heart is one of the sensitive organs to microwave radiation. Studies have shown that microwave radiation can cause damage to the structure and function of the heart, manifested as disordered arrangement of muscle fibers in myocardial tissue, dissolution and breakage of some myofilaments, and disturbances in myocardial enzyme spectrum such as LDH. In addition, ROS is a chemically active oxygen-containing atom or atomic group. Its large-scale production and accumulation can cause peroxidation of unsaturated fatty acids in lipids, destroy t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61P9/00A23L33/105
Inventor 彭瑞云赵黎张雪岩张静姚斌伟徐新萍董霁胡宗倩周红梅
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA