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Novel compound of gallwood capable of promoting endothelial injury repair and its preparation method and application

An endothelial injury and compound technology, which is applied in the field of compounds to achieve the effects of being convenient to take, promoting proliferation, and promoting endothelial injury repair

Active Publication Date: 2021-06-01
HAINAN SENQI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is no report to isolate the bile wood compound with resistance to endothelial damage repair from the bile wood

Method used

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  • Novel compound of gallwood capable of promoting endothelial injury repair and its preparation method and application
  • Novel compound of gallwood capable of promoting endothelial injury repair and its preparation method and application
  • Novel compound of gallwood capable of promoting endothelial injury repair and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. A new compound of bile wood that promotes cell repair, which is prepared by the following method:

[0036] (1) Take the stem of the gallwood, pulverize it, add water of 6 times the volume of the weight of the gallwood, soak and extract in 3 times, each time for 5 hours, combine the extracts, and concentrate to obtain the extract;

[0037] (2) Get the medicinal extract obtained in step (1), add dichloromethane and ethyl acetate of 3 times the volume of the medicinal extract weight to extract, extract respectively to obtain dichloromethane and ethyl acetate extraction parts; get the ethyl acetate part, Put on a silica gel chromatographic column, elute and separate with a dichloromethane-methanol elution solvent with a volume ratio of 95:5-85:15-70:30-50:50-10:90, and analyze and merge similar parts by TLC, Then use the preparative liquid phase to separate and elute with methanol at a volume concentration of 60% to obtain a new compound of choline (16-dehydroxymethylcho...

Embodiment 2

[0046] Take 16-dehydroxymethylcholigyn B and add purified water to dissolve it, add an appropriate amount of stevioside, adjust the pH to 5.5, subpackage, and sterilize to obtain the product.

Embodiment 3

[0048] Take 16-dehydroxymethylcholanine B, add dextrin and starch, mix evenly, granulate, and then pack into capsules to make capsules.

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PUM

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Abstract

The present invention discloses a novel compound of bilewood alkaloids capable of promoting the repair of endothelial damage. The compound is prepared by the following method: taking the stem of bilewood, pulverizing it, adding water for soaking and extracting, collecting the extract, concentrating under reduced pressure to obtain the bilewood extract Cream, the extract is extracted to obtain the dichloromethane part and ethyl acetate part, take the ethyl acetate part, put it on a silica gel column, and use dichloromethane-methanol with a volume ratio of 95:5 to 10:90 as the eluting solvent , combined similar parts by TLC analysis, and then applied to the preparative liquid phase, using methanol water as the eluent, prepared by liquid chromatography to obtain the new compound of bile wood. Based on the mechanism of cell repair, the present invention conducts in-depth research on the proliferative activity of the new compound of eucalyptus under the guidance of the theory. Experimental studies show that the new compound of eucalyptus can significantly promote the proliferation of HUVEC cells and the repair of endothelial cell scratches. It can be used to prepare medicines for preventing and treating inflammatory endothelial injury.

Description

technical field [0001] The present invention relates to a compound isolated from the eucalyptus eucalyptus, in particular to a new compound of the eucalyptus eucalyptus which can promote the repair of endothelial damage. Background technique [0002] Nauclea officinalis Pierre ex Pitard. is a plant of Rubiaceae ebony, also known as ebony, bear bile tree, yellow bile wood, mountain bear bile, etc. It has the effects of clearing heat and detoxification, reducing swelling and pain, and is used to treat throat Inflammation, bronchitis, acute tonsillitis embolism. The alkaloids of bilewood have various pharmacological effects, including alleviating LPS-induced inflammation, antitumor activity, etc. [0003] Endothelial cell injury repair is an important link in the recovery of infectious diseases. Promoting endothelial cell proliferation can accelerate the repair of endothelial injury induced by various reasons, thereby promoting the healing of infectious diseases in the body. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/14A61P29/00A61P11/00A61P11/04A61P17/02A61K31/4375
CPCA61P11/00A61P11/04A61P17/02A61P29/00C07D471/14
Inventor 李永辉张俊清张旭光李海龙赖伟勇麦世瑛吴娇霞
Owner HAINAN SENQI PHARMA CO LTD