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Preparation method of wake robin extracts and saponins of wake robin extracts and application of wake robin extracts and saponins to preparation of medicine for resisting ischemic heart disease

A technology of trillium saponin and trillium, applied in the field of traditional Chinese medicine preparation

Active Publication Date: 2016-01-13
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above studies have not been reported yet. Through systematic in vivo and in vitro experimental studies, we first found that it has a good therapeutic effect on ischemic heart disease

Method used

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  • Preparation method of wake robin extracts and saponins of wake robin extracts and application of wake robin extracts and saponins to preparation of medicine for resisting ischemic heart disease
  • Preparation method of wake robin extracts and saponins of wake robin extracts and application of wake robin extracts and saponins to preparation of medicine for resisting ischemic heart disease
  • Preparation method of wake robin extracts and saponins of wake robin extracts and application of wake robin extracts and saponins to preparation of medicine for resisting ischemic heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018]A trillium extract and pinanogenin-3-O-α-L-rhamnopyranosyl (1→2)-β-D-glucoside, pinanogenin-3-O- α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucoside and pinanogenin-3-O- α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]- The preparation method of β-D-glucoside: grind Trillium herbaceae into coarse powder, soak in 70% ethanol for 1 hour, heat and reflux extraction with 10 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 65°C to 1.0g raw medicinal materials / ml, refrigerated overnight at 0-4°C, gelatinized, filtered, and the filtrate was extracted with an organic solvent such as n-butanol, and the organic solvent was recovered to obtain the extract and suspended in water. Enriched with AB-8 macroporous adsorption resin, eluted with water, 30% ethanol, 60% ethanol and 85% ethanol in sequence to obtain the eluted parts, drie...

Embodiment 2

[0020] A trillium extract and pinanogenin-3-O-α-L-rhamnopyranosyl (1→2)-βD-glucoside, pinogenin-3-O-α- L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-βD-glucoside and pinanogenin-3-O-α-L- Rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-βD-glucoside The preparation method of the medicinal material is: crush Trillium herbaceae into coarse powder, soak in 60% ethanol for 2.0 hours, heat and reflux extraction with 12 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 70°C to 1g raw medicinal materials / ml, refrigerated overnight at 0-4°C, gelatinized, filtered, enriched with D101 macroporous adsorption resin on the filtrate, water, 30% ethanol, 60% ethanol and 85% ethanol in sequence % ethanol for elution to obtain the eluted parts, dry them by spray drying, and collect the spray-dried powder to obtain the final product. Among them, 85% of the ...

Embodiment 3

[0022] A trillium extract and pinanogenin-3-O-α-L-rhamnopyranosyl (1→2)-β-D-glucoside, pinanogenin-3-O- α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucoside and pinanogenin-3-O- α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]- The preparation method of β-D-glucoside: grind Trillium herbaceae into coarse powder, soak in 50% ethanol for 1.0 hour, heat and reflux extraction with 8 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 70°C to 1g raw medicinal materials / ml, refrigerated overnight at 0-4°C, analyzed gel, filtered, enriched with HPD400 macroporous adsorption resin on the filtrate, water, 30% ethanol, 50% ethanol and 85% ethanol in sequence % ethanol for elution to obtain the eluted parts, dry them by spray drying, and collect the spray-dried powder to obtain the final product. Among them, 85% of the eluted parts are ma...

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Abstract

The invention relates to a preparation method of wake robin extracts and saponins of wake robin extracts and application of wake robin extracts and saponins in preparation of medicine for resisting ischemic heart diseases. Prepared wake robin extracts are 85% ethyl alcohol eluting parts of large-hole adsorption resin of wake robin total extracts, and the total saponin content of wake robin extracts is larger than 90%, and wake robin extracts are mainly prepared from 66-75% of pennogenin, 3-7% of wake robin saponin and 5-8% of dioscin. Prepared wake robin saponin compounds include one or more of pennogenin-3-O-alpha-L-pyran rhamanopyranosyl(1-2)-beta-D-glucoside, pennogenin-3-O-alpha-L-pyran rhamanopyranosyl(1-4)-[alpha-L-pyran rhamanopyranosyl(1-2)]-beta-D-glucoside and pennogenin-3-O-alpha-L-pyran rhamanopyranosyl(1-4)-alpha-L-pyran rhamanopyranosyl(1-4)-[alpha-L-pyran rhamanopyranosyl(1-2)]-beta-D-glucoside and have similar treatment functions in human bodies due to the sharing of structural units of pennogenin-3-O-alpha-L-pyran rhamanopyranosyl(1-2)-beta-D-glucoside and the like.

Description

technical field [0001] The invention relates to a preparation method of a trillium extract and a saponin compound therein and its application in the preparation of anti-ischemic heart disease drugs, belonging to the technical field of traditional Chinese medicine preparation. Background technique [0002] Trillium is Trillium tschonoskii Maxim., a plant of the genus Trillium in the family Liliaceae, commonly known as a bead on the top of the head, taro seven, lion seven, etc. It is a traditional precious Chinese medicine with the effect of prolonging life. Indications of dizziness, insomnia, bruises, traumatic bleeding, neurasthenia, hypertension, sequelae of concussion and other diseases, is one of the famous folk medicines of Enshi Tujia. At present, domestic and foreign studies on the pharmacological activity of Trillium plants have shown that Trillium has strong anti-inflammatory, immune regulation, improving learning and memory functions of rats, and anti-aging effects....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K31/7048A61P9/10
Inventor 张忠立左月明
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE