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Manufacturing method for acellular dermal matrix tympanic membrane and external ear tissue repair material

A decellularized dermis and tissue repair technology, applied in medical science, prostheses, etc., can solve the problems that the sound air conduction function of the tympanic membrane is greatly affected, and the repairing effect of the tympanic membrane repair material is poor, so as to broaden the source of raw materials, improve the clinical effect, cost reduction effect

Active Publication Date: 2016-01-13
BEIJING QINGYUAN WEIYE BIO TISSUE ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] It can be seen that the repair effect of the tympanic membrane repair material in the prior art is poor, and it can only play a role of barrier, which has a great impact on the original sound and air conduction function of the tympanic membrane. It is urgent to develop a new preparation method of the tympanic membrane repair material. To overcome the shortcomings of current methods of manufacturing eardrum repair materials

Method used

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  • Manufacturing method for acellular dermal matrix tympanic membrane and external ear tissue repair material
  • Manufacturing method for acellular dermal matrix tympanic membrane and external ear tissue repair material
  • Manufacturing method for acellular dermal matrix tympanic membrane and external ear tissue repair material

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preparation example Construction

[0040] The preparation method of the decellularized dermal matrix tympanic membrane and external ear tissue repair material of the present invention comprises the following steps:

[0041] Step A: Select the dermis of a mammalian body or a human body.

[0042] Mammals are pigs, cattle, sheep, etc. Preferably, the dermis on the human body is selected. The structure and function of the dermis in different parts of mammals are different, and the types of matrix proteins and various factors contained in different parts of the dermis, The quantity varies. According to the structure, function and content of various factors in different parts of the dermis, the corresponding parts of the dermis are selected in a targeted manner.

[0043] Step B: by comparing the structures of the dermal tissue and the tympanic membrane tissue, determine the α-layer structure of the dermal tissue structure which is similar to the structure of the tympanic membrane tissue and adjacent to 3 layers;

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Embodiment 1

[0063] Using the acellular dermal matrix tympanic membrane and external ear tissue repair material patching method of the present invention, 28 ears of guinea pigs on the outer surface of the perforated tympanic membrane were patched, and 25 ears were closed 2 weeks after the operation, and the tympanic membrane was congested and thickened. Infection occurred in 3 ears. After 2 weeks, the hyperemia of the tympanic membrane gradually subsided, and at 6 weeks, part of the eardrum was free of hyperemia, transparent, smooth, and vascular hyperplasia was visible on the surface.

[0064] please cooperate figure 1 As shown, the tympanic membrane was congested and thickened 2 weeks after the acellular dermal matrix tympanic membrane was subsidized and reconstructed (↓), ×40. Please refer to figure 2 As shown, 6 weeks after the acellular dermal matrix tympanic membrane was subsidized to reconstruct the tympanic membrane, the tympanic membrane was grayish white with a smooth surface ...

Embodiment 2

[0067] Please refer to Figure 12 As shown, the surface of the external ear tissue repair material of the present invention is smooth before transplantation, and a large number of intertwined collagen fibers (→) can be seen without any cell attachment. Scale bar: 30 μm. Please refer to Figure 13 As shown, before the implantation of the external ear tissue repair material of the present invention, there are a small amount of papillary structures on the local surface (→), and a large number of intertwined collagen fibers can be seen without any cell attachment. Scale bar: 30 μm. Please refer to Figure 14 As shown, on the surface of the normal tympanic membrane and the external auditory canal, squamous epithelial desquamation (→) and demyelinated squamous epithelial cells (▲) can be seen, the scale bar: 30 μm. Please refer to Figure 15 As shown, 8 weeks after the implantation of the external ear tissue repair material of the present invention, collagen fibers can be seen on...

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Abstract

The invention discloses a manufacturing method for an acellular dermal matrix tympanic membrane and an external ear tissue repair material. The method comprises the following steps of A, selecting mammal body or human body dermis tissue; B, determining three adjacent layers of dermis tissue alpha layer structures similar to the structure of tympanic membrane tissue in the dermis tissue structure by comparing the dermis tissue with the tympanic membrane tissue structure; C, removing the other layers except for the dermis tissue alpha layer structures in the dermis tissue structure to obtain a dermis tissue alpha layer preliminary material. According to the specificity of the dermis characteristics and the tympanic membrane tissue structure for development and improvement, and the tympanic membrane repair material similar to the human body tympanic membrane structure and the tissue characteristics is obtained. On the basis that the tissue integrity repair is completed, the tympanic membrane function is basically repaired, and the clinic effect is greatly improved; the raw material sources of the material for manufacturing the acellular dermal matrix tympanic membrane and the external ear tissue repair material are broadened, and the cost for manufacturing the repair material is lowered.

Description

technical field [0001] The invention relates to a preparation method of decellularized dermal matrix tympanic membrane and external ear tissue repair materials. Background technique [0002] At present, autologous temporalis muscle fascia is mainly used for repairing the tympanic membrane clinically, and animal-derived biomaterials are also used for repairing. Due to the problems of secondary damage and scarring of ectopic tissue, the repair effect is not satisfactory. The structure of fascia tissue and tympanic membrane tissue is quite different, so in terms of repair results, only the barrier function of the outer ear and middle ear is completed, and the repair is a scar repair, which has a great impact on the original sound air conduction function of the tympanic membrane. [0003] It can be seen that the repair effect of the tympanic membrane repair material in the prior art is poor, and it can only play a role of barrier, which has a great impact on the original sound a...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 张小明李燕青
Owner BEIJING QINGYUAN WEIYE BIO TISSUE ENG
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