Non-degeneration plant holoprotein extracting solution and preparing method thereof

Abstract

The invention relates to plant protein extracting, in particular to a non-degeneration plant holoprotein extracting solution and a preparing method thereof. The extracting solution is characterized by being an aqueous solution containing a phosphate buffer solution, EDTA, glycerol, PVP and protease inhibitors. The extracting solution has the advantages that plant holoprotein extracted with the holoprotein extracting solution is suitable for analyzing the activity of protease and measuring the protein concentration with methods such as BCA; the holoprotein is not degenerated, reducing agents influencing measuring of the BCA and the like are not added, the extracted holoprotein can be stored for several months in the frozen mode, and sediment is basically avoided after re-melting; in addition, the activity of the holoprotein is basically free of influences before and after storage, the extracted holoprotein is high in amount and can be further directly used for further separation purification, and therefore the extracting solution has important application value in the aspect of plant physiology and biochemistry researching.

Description

technical field [0001] The invention relates to plant protein extraction and belongs to the technical field of plant physiology and biochemistry, in particular to a non-denatured plant whole protein extract and a preparation method thereof. Background technique [0002] The study of plant physiology and biochemistry is inseparable from the detection of related enzyme activities, such as the changes of SOD enzyme activity and GST enzyme activity that are often concerned under different stress conditions. Due to the specificity of different enzyme activities, the use of whole protein extracts for detection does not require purification of the target protein, which is time-saving, labor-saving, and reliable. At present, there are many plant protein extracts on the market, which are generally used in subsequent proteomics research, and are characterized by the addition of urea, DTT, etc. Urea denatures proteins, making enzyme activity analysis impossible; and reducing agents su...

AI Technical Summary

Problems solved by technology

Urea denatures proteins, making it impossible to analyze enzyme activity; while reducing agents such as DTT seriously interfere with BCA, Bradford, and other methods for measuring protein concentration, making accurate quantitative comparisons of enzyme activities impossible

When many laboratories extract whole plant protein for physiological and biochemical analysis, in order to avoid protein denaturation and degradation, it is necessary to analyze it immediately after extraction, which is obviously unrealistic for large quantities of samples

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