Method used for detecting fruit pesticide residue

A technology for pesticide residues and fruits, applied in the field of detection of pesticide residues in fruits, can solve the problems of complex detection process, harsh experimental conditions, and large initial investment, and achieve good sensitivity and linear range, high precision and repeatability, and meet the detection requirements. desired effect

Inactive Publication Date: 2016-01-13
广西南宁民安检测技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the instrumental analysis detection method has the highest accuracy, but its detection process is complicated and the detection ti

Method used

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  • Method used for detecting fruit pesticide residue

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Embodiment 1

[0015] Embodiment 1: The present invention provides a method for detecting fruit pesticide residues, comprising the following steps: Step a: Rinse the sample to be detected with clear water and cut it into 4*4mm block fragments;

[0016] Step b: Take 5g-15g fragment samples and add them to a centrifuge tube containing anhydrous sodium acetate and anhydrous magnesium sulfate, then add acetic acid acetonitrile solution, vibrate vigorously, perform ultrasonic extraction, and then centrifuge;

[0017] Step c: Put the acetonitrile extract obtained after centrifugation into a centrifuge tube containing 2-5g of purifying agent, place the centrifuge tube on a shaker for 5 minutes, let it stand for 5 minutes, draw the upper layer solution, and directly insert it into the syringe type Filter, filter to remove suspended matter in the solution, and collect the filtrate in a test tube;

[0018] Step d: place the test tube containing the filtrate in a water bath with a temperature of 40 deg...

Embodiment 2

[0021] Other steps are the same as in Example 1. In step b, take 10 g of fragment samples, add them to a 60 ml centrifuge tube containing 2 g of anhydrous sodium acetate and 8 g of anhydrous sodium sulfide, then add 20 ml of 1% acetic acid acetonitrile solution, shake vigorously for 2 min, and ultrasonically After extraction for 20 min, centrifuge at 5000 r / min for 2 min. In said step c, the cleaning agent is potassium persulfate.

Embodiment 3

[0023] Other steps are the same as in Example 1. In step b, take 5 g of fragment samples, add them to a 60 ml centrifuge tube containing 2 g of anhydrous sodium acetate and 8 g of anhydrous sodium sulfide, then add 20 ml of 1% acetic acid acetonitrile solution, shake vigorously for 2 min, and ultrasonically After extraction for 20 min, centrifuge at 5000 r / min for 2 min. In said step c, the cleaning agent is potassium persulfate.

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Abstract

The invention belongs to the technical field of food safety, and more specifically relates to a method used for detecting fruit pesticide residue. The method comprises following steps: step a, a sample to be detected is washed with clear water, and is cut into 4*4mm blocks; step b, 5 to 15g sample block is added into centrifugal tubes filled with anhydrous sodium acetate and anhydrous magnesium sulfate, an acetic acid acetonitrile solution is added, an obtained mixture is subjected to violent shaking, ultrasonic extraction, and centrifugation; step c, an acetonitrile extract obtained via centrifugation is delivered into centrifugal tubes filled with 2 to 5g of a cleaning agent, the centrifugal tubes are subjected to vibration using an oscillator for 5min, and are allowed to stand for 5min, a supernatant is collected, is delivered into a needle cylinder-type filter directly so as to remove suspended matters in the supernatant via filtering, and an obtained filtrate is collected in a test tube; step d, the test tube filled with the filtrate is subjected to water bath at 40DEG C, and the solvents in the test tube are removed completely via air purging, and an obtained mixed solution with an acetonitrile/water volume ratio of 1:1 is diluted to a constant volume for further detection; and step e, liquid chromatography is adopted for detection. The method is rapid, accurate, high in efficiency, and low in cost.

Description

technical field [0001] The invention relates to the technical field of food safety, in particular to a method for detecting fruit pesticide residues. Background technique [0002] Pesticides are used to kill insects, fungi, and other organisms that harm crop growth. The earliest pesticides used were DDT, BCH, etc., which can eliminate pests in large numbers. However, they have good stability, can exist in the environment for a long time, and accumulate continuously in animals, plants and humans, so they are eliminated. Later, organophosphorus pesticides, such as dichlorvos, were used instead of the original pesticides. Then they are too toxic and very harmful to humans and animals. In recent years, a group of high-efficiency and low-toxicity pesticides have appeared, and now people have found specific pesticides, namely hormone pesticides. [0003] However, no matter how advanced pesticides are, they belong to the category of chemicals after all, which will affect the en...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 杨建国
Owner 广西南宁民安检测技术有限公司
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