Preparation method of hypolipidemic compound Fuzhuan tea element A

A kind of technology of pochacin and compound, applied in the field of hypolipidemic compound and preparation thereof

Active Publication Date: 2016-01-20
清云凤凰茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is not yet a mature prepara

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  • Preparation method of hypolipidemic compound Fuzhuan tea element A
  • Preparation method of hypolipidemic compound Fuzhuan tea element A
  • Preparation method of hypolipidemic compound Fuzhuan tea element A

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preparation of Fuchalin A

[0028] Weigh 500g (dry weight) of sun-dried green maocha, fill it with tide water until the water content is 30-35%, pack in 500ml triangular flasks, sterilize at 121°C for 20min, insert Aspergillus niger into the tea leaves, and inoculate 5×10 6 Spores / g, cultured in a biochemical incubator at 28°C, after 15 days, take out the tea sample and dry it in an oven at 50°C until the moisture content is 6%; crush the tea sample through a 60-mesh sieve; weigh 250g of crushed tea leaves, add tea leaves The volume concentration of five times the weight is 80% methanol, use an ultrasonic cleaner to vibrate and leach twice under the conditions of 50°C, 50Hz, and 120w, each time for 1h, filter, and combine the filtrate; the filtrate after leaching is rotated under reduced pressure Concentrate with a concentrator at 40°C to obtain concentrated solution 1 (50ml); column chromatography for separation and purification, select a 7cm inner diameter silica gel ...

Embodiment 2

[0030] Preparation of Fuchalin A

[0031] Weigh 1000g (dry weight) of sun-dried green maocha, fill it with tide water until the water content is 28-30%, pack in 500ml triangular flasks, sterilize at 115°C for 28min, insert Aspergillus niger into the tea leaves, and inoculate 2×10 7 Spores / g, cultured in a biochemical incubator at 28°C, after 21 days, take out the tea sample and dry it in an oven at 60°C to a moisture content of 5%; crush the tea sample through a 60-mesh sieve; weigh 350g of crushed tea leaves, add tea leaves The volume concentration of eight times the weight is 80% methanol, use an ultrasonic cleaner to vibrate and leach twice under the conditions of 50°C, 60Hz, and 100w, each time for 1h, filter, and combine the filtrate; the filtrate after leaching is rotated under reduced pressure Concentrate with a concentrator at 40°C to obtain concentrated solution 1 (50ml); column chromatography for separation and purification, select a chromatographic column with an in...

Embodiment 3

[0033] Preparation of Fuchalin A

[0034] Weigh 2000g (dry weight) of sun-dried green maocha, fill it with tide water until the water content is 26-28%, pack in 500ml triangular flasks, sterilize at 125°C for 30min, insert Aspergillus niger into the tea leaves, and inoculate 5×10 6Spores / g, cultured in a biochemical incubator at 28°C, after 20 days, take out the tea sample and dry it in an oven at 55°C to a moisture content of 4%; crush the tea sample through a 50-mesh sieve; weigh 550g of crushed tea leaves, add tea leaves The volume concentration of 10 times the weight is 80% methanol, use an ultrasonic cleaner to vibrate and leach twice under the conditions of 50°C, 60Hz, and 150w, each time for 1h, filter, and combine the filtrate; the filtrate after leaching is rotated under reduced pressure Concentrate with a concentrator at 40°C to obtain concentrated solution 1 (50ml); column chromatography for separation and purification, select a chromatographic column with an inner ...

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Abstract

The invention discloses a preparation method of a hypolipidemic compound Fuzhuan tea element A and application of the compound in reducing the blood fat. Aspergillus niger is inoculated to ferment tealeaves, and the Fuzhuan tea element A is purified and prepared by virtue of technologies such as column chromatography, extraction and the like. The method comprises the following steps: (1) fermenting; (2) crushing tealeaves; (3) extracting by utilizing a methanol solvent with the volume concentration of 80 percent; (4) concentrating filtrate; (5) separating and purifying by virtue of column chromatography: selecting 7cm, 5.5cm and 3cm chromatographic columns to be filled with silica gel and sephadex gel, and separating; (6) adding acetonitrile to extract; (7) freeze drying: freezing the collected supernatant in a refrigerator, and freeze drying by utilizing a freeze drying machine. The compound Fuzhuan tea element A is high in purity and can be used for preparing a hypolipidemic drug.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a blood lipid-lowering compound and its preparation method and application. Background technique [0002] Hyperlipidemia (hyperlipidemia, HLP) is a disorder of lipid metabolism in the human body, and one or more lipid components in the plasma are abnormally increased, manifested as hypercholesterolemia, hypertriglyceridemia or both. both. Hyperlipidemia can induce atherosclerosis, cardiovascular and cerebrovascular diseases, such as hypertension, coronary heart disease, cerebrovascular disease, senile dementia, etc. Therefore, the harm of hyperlipidemia has become a worldwide concern. [0003] Teadenol A (Teadenol A) was discovered and named by Japanese scholars in Japanese fermented tea, and has significant lipid-lowering effects (WulandariRA, AmanoM, YanagitaT, TanakaT, KounoI, KawamuraDandIshimaruK. Newphenolic compounds from Camelliasinensis L. leavesfermented with Aspergillus sp...

Claims

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Application Information

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IPC IPC(8): C07D493/04A61P3/06
CPCC07D493/04
Inventor 赵明苏小琴马燕陈茂李家华陈盛虎段双梅万晶琼张高菊
Owner 清云凤凰茶业有限公司
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