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Lead ion visual detection method and detection kit

A technology for detecting kits and lead ions, which is applied in the field of environmental analytical chemistry, can solve the problems of poor stability and high cost, and achieve the effects of low cost, simple operation and high detection sensitivity

Active Publication Date: 2016-01-20
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, potential disadvantages require synthesis of RNA-containing DNA for detection, poor stability, and high cost

Method used

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  • Lead ion visual detection method and detection kit

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Embodiment 1

[0063] A lead ion visual detection kit comprises the following components:

[0064] (1) Nucleic acid sequences A, B, C, D; one end of A is modified with biotin; one end of C is modified with sulfhydryl group (-SH); one end of D is modified with biotin. The specific sequence is as follows:

[0065] Nucleic acid sequence A: 5'-GGGTGGGT(a)GGGTGGGT(b)-3'(SEQ ID NO: 1);

[0066] Nucleic acid sequence B: 5'-biotin-AAAAAA-ACCCACCC(b*)TAT(c)ACCCACCC(a*)-3'(SEQ ID NO: 2);

[0067] Nucleic acid sequence C: 5'-SH-AAAAAA-GGGTGGGT(a)ATA(c*)-3'(SEQ ID NO: 3);

[0068] Nucleic acid sequence D: 5'-biotin-AAAAAA-TAT(c)ACCCACCC(a*)-3' (SEQ ID NO: 4).

[0069] (2) Lead ion standard solution.

[0070] (3) Test strip detection platform: including sample pad, gold standard pad, nitrocellulose membrane and absorbent paper, these four parts are fixed on the rubber plate in turn from left to right. C-modified gold nanoparticles, sprayed on the gold standard pad, the detection area of ​​the nitroc...

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Abstract

The invention discloses a lead ion visual detection method and detection kit. The detection kit comprises a test strip detection platform, a buffer system and nucleotide sequences A, B C and D. By means of the characteristics that the A, B, C and D are partially complementary in sequence and the A has a lead ion specific recognition element, A-B heteroduplex and lead ion recognition are utilized, the A and lead ions form a G-tetramer structure, and the A is dissociated out; then the B-chain obtained through replacement is dripped onto a sample pad of the test strip for detection, and the B forms a macroscopic strip in the detection area by continuing to be combined with the C. Neither traditional 8-17DNAzyme nor RNA synthesis nor antibodies are needed, the detection result is directly macroscopic, operation is easy, cost is low, and the lead ion visual detection method and detection kit are suitable for being used and popularized in units. The lead detection limit of the method is 1 nM which is lower than 72 nM, the maximum allowable limit, ruled by the USEPA, of lead ions in drinking water.

Description

technical field [0001] The invention belongs to the field of environmental analytical chemistry and relates to a lead ion detection method and a detection kit. Background technique [0002] Lead ions are seriously harmful to the human body and are an important indicator for environmental monitoring. Its main toxic effect is to cause anemia, neurological dysfunction and kidney damage, reproductive system damage and so on. The U.S. Environmental Protection Agency stipulates that the maximum allowable amount of lead ions in drinking water must not exceed 72nM. At present, the routine detection methods of trace lead ions in the environment mainly include atomic absorption method, atomic fluorescence spectrometry, inductively coupled plasma emission spectrometry, and electrochemical methods. However, these methods are cumbersome to operate, require cumbersome pretreatment, specialized analytical technicians, and expensive instruments, which are not conducive to rapid on-site an...

Claims

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Application Information

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IPC IPC(8): G01N33/00
Inventor 陈俊华周顺桂
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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