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Quantum dot-based multi-level detection marker sensor and preparation method thereof

A technology of quantum dots and sensors, which is applied in the detection and monitoring of autoimmune diseases, nanomaterial preparation and immunohistochemical technology, can solve the problems of cumbersome preparation process, limitations, a large number of reagents and clinical specimens, etc., to achieve high biological activity, High stability, narrow emission wavelength effect

Active Publication Date: 2017-05-31
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the detection methods of autoantibody antigens and their analogues are mainly western blotting and ELISA (enzyme-linked immunosorbent adsorption), etc., but these methods have cumbersome preparation processes without exception, and because they require a relatively large amount of reagents and clinical specimens. Restricted and not capable of multi-level detection

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  • Quantum dot-based multi-level detection marker sensor and preparation method thereof
  • Quantum dot-based multi-level detection marker sensor and preparation method thereof
  • Quantum dot-based multi-level detection marker sensor and preparation method thereof

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Embodiment 1

[0052] Preparation method of quantum dot-based sensor for multi-level detection of autoimmune disease biomarkers:

[0053] 1) Synthesis of CdTe-ZnS core-shell quantum dots.

[0054] The synthesis steps of CdTe-ZnS core-shell quantum dots are as follows: Weigh 12.69 mg of potassium tellurite and prepare a solution as tellurium precursor, 53.306 mg of hydrated cadmium acetate as a source of cadmium, add mercaptopropionic acid in proportion as coating solution 29.6 μL, Adjust the pH of the system to 10.5-11, continue to reflux and heat for 1 hour, and synthesize CdTe QDs with an emission peak of 518 nm by using a high-temperature hydrothermal method. Measure 10mL of CdTe QDs, and blow nitrogen gas for 20min to ensure that it is in an oxygen-free environment. Add 10mL solution made of 3.512mg zinc acetate dihydrate, then add 3.84mg sodium sulfide, also use 3.4μL mercaptopropionic acid as the coating solution, readjust the pH of the system at 10.5-11, continue to pass nitrogen, an...

Embodiment 2

[0067] The preparation process of the quantum dot-based multi-level detection autoimmune disease biomarker sensor specifically includes the following steps:

[0068] 1) Synthesis of CdTe-ZnS core-shell quantum dots.

[0069] The synthesis steps of CdTe-ZnS core-shell quantum dots are as follows: Weigh 12.69 mg of potassium tellurite and prepare a solution as tellurium precursor, 53.306 mg of hydrated cadmium acetate as a source of cadmium, add mercaptopropionic acid in proportion as coating solution 29.6 μL, Adjust the pH of the system to 10.5-11, continue to reflux and heat for 6 hours, and use high-temperature hydrothermal synthesis to obtain 621nm CdTe QDs for future use. Measure 10mL of CdTe QDs, and pass nitrogen gas for 20min to ensure that it is in an oxygen-free environment. Add 10mL solution made of 3.512mg zinc acetate dihydrate, then add 3.84mg sodium sulfide, also use 3.4μL mercaptopropionic acid as the coating solution, readjust the pH of the system at 10.5-11, c...

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Abstract

The invention discloses a quantum dot-based multi-stage autoimmune disease biomarker detection sensor and a preparation method thereof. The preparation method of the sensor comprises the following steps: combining CdTe-ZnS core-shell quantum dots with different emission peaks with an antigen so as to form a QDs-Ag compound with multiple optical signals, loading the compound to a silylated glass substrate, detecting a target by virtue of immunological recognition between the antigen and a biomarker, further forming fluorescence resonance energy transfer between QDs and AuNPs by virtue of connection of a second antibody-DNA-Au NPs compound, and generating change of the optical signals. The CdTe-ZnS core-shell quantum dots are synthesized by utilizing a hydrothermal method, and the second antibody-DNA-Au NPs compound is obtained by adopting a biological cross-linking method. According to the sensor disclosed by the invention, the multi-stage detection of autoimmune disease biomarkers can be finished by virtue of wide-excitation and narrow-emission fluorescence properties of the quantum dots.

Description

technical field [0001] The invention belongs to the interdisciplinary field of nanomaterial preparation and immunohistochemical technology, and relates to a sensor and a manufacturing method thereof, in particular to an optical sensor for detecting autoimmune disease biomarkers combined with quantum dots and an immune probe and a preparation method thereof, which is applied to detection and monitoring autoimmune disease technical field. Background technique [0002] Compared with traditional fluorescent dyes, quantum dots have the following advantages: (1) good photostability, resistance to bleaching, fluorescence intensity is about 20 times that of rhodamine 6G, and stability is about 100 times; (2) excitation The spectral range is wide and multiple components can be excited at the same time; (3) The half-peak width of the emission spectrum is relatively narrow, the peak shape is sharp, the symmetry is good, and the emission spectrum is adjustable. The adjustable wavelength...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57484
Inventor 温永强庞铎周雅彬雷霞杨志林张学记
Owner UNIV OF SCI & TECH BEIJING