Freeze preservation of living cells

A cryoprotectant, freezer technology, applied in the preservation of plants, preservation of human or animal bodies, plant cells, etc.

Inactive Publication Date: 2016-01-20
UNIV DE NAMUR
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, current methods often require expensive liquid nitrogen, specialized devices to provide programmable cooling rates, and high molar concentrations of cryoprotectants that can be cytotoxic

Method used

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  • Freeze preservation of living cells
  • Freeze preservation of living cells
  • Freeze preservation of living cells

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Embodiment Construction

[0028] The present invention provides a simple, rapid and inexpensive method for cryopreservation of (plant, including algae) cells, genetically modified (plant) cells, organelles or tissue cultures. This technology is based on the protective properties of the silica matrix at low temperatures (-30°C or lower to -196°C, preferably -70°C or lower to -196°C), which can be used as an original, simple and effective technique For cryopreservation of (plant, including algae) cells, genetically modified (plant) cells, organelles or tissue lines in common laboratory freezers.

[0029] Silicate (SiO 2 .nH 2 The O) form occurs naturally in soil. These mineral phases are weathered in water to produce monomeric orthosilicic acid (Si(OH) 4 ) and some disilicate acids, which are absorbed, transported, and deposited throughout the plant as amorphous silica, especially in the cell walls rather than in the plant's vacuoles. Several reports have highlighted that these Si species can increas...

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Abstract

The present invention is related to a method for the cryopreservation of a (plant) cell, (plant) organelle or (plant) tissue, where said (plant) cell, (plant) organelle or (plant) tissue is encapsulated in a matrix formed of an abiotic material in presence of one or more additives with cryoprotectant effect. The method of the invention provides a faster, more effective and cheaper way to cryopreserve biological material.

Description

technical field [0001] The present invention relates to the field of cryopreservation (cryopreservation) of cells, in particular plant cells, (plant) organelles and (plant) tissues including algae. Background technique [0002] Plant cell and tissue culture are important tools widely used in basic and applied research. They allow high-throughput studies such as gene function and regulation, metabolic analysis, or biopesticide (biopesticide) development. They are also attractive and powerful biological mechanisms for producing fine chemicals in bioreactors. In particular, plant cell culture is of interest for the production of properly glycosylated and folded pharmaceutically active proteins (eg, immunoglobulins, interleukins). They are intrinsically safe, in contrast to mammalian or microbial production platforms, as they are not host human pathogens and do not produce endotoxins. However, maintaining multiple wild-type and transgenic cell lines in various laboratories in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0231A01N3/00C12N5/04
Inventor 克里斯托夫·默尼尔皮埃尔·范 卡策姆巴奥-利安·苏
Owner UNIV DE NAMUR
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