Detection method and kit for colorectal cancer cells based on quantum dot labeling

Abstract

The invention discloses a detection method and a kit for colorectal cancer cells based on quantum dot labeling. The detection method comprises steps of an anti-human M2-type pyruvate kinase monoclonal antibody preparation process, a quantum dot fluorescent probe preparation process and a process that quantum dot fluorescent probes are added in colorectal cancer cells and of fluorescence images of the colorectal cancer cells are observed under a confocal fluorescence microscope. Through anti-human-M2-type-pyruvate-kinase monoclonal antibodies labeled with quantum dots, a liquid chip or an immunofluorescence detection colorectal cancer cell or protein immunologic diagnosis kit can be formed. The kit has advantages of high sensitivity and high specificity, rapid and accurate detection can be achieved, secreta comprising feces and serum can be detected, and detection accuracy of the colorectal cancer can be raised greatly.

Description

technical field [0001] The invention relates to the technical field of detection methods for colorectal cancer cells, in particular to a detection method for colorectal cancer cells based on quantum dot labels. [0002] The present invention also relates to the detection kit technology of colorectal cancer, especially the kit containing monoclonal antibody 33.26 and monoclonal antibody 31.3. Background technique [0003] Colorectal cancer is one of the three most common malignant tumors in the world, and its mortality rate ranks third. In recent years, with the improvement of people's living standards and changes in diet structure, its incidence has shown an obvious upward trend. However, the early diagnosis of colorectal cancer at home and abroad is still at a low level, and about half of the patients have entered the middle and late stages of colorectal cancer when they are diagnosed. Therefore, improving the early diagnosis of colorectal cancer is a key issue that urgent...

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Problems solved by technology

Because it is an invasive examination with dangerous side effects such as bleeding and intestinal perforation, certain equipment and instruments are required, and the professional level of the operator is high, so there are great limitations in the examination of a large number of people.

[0006] (3) Commonly used tumor markers detected by electrochemiluminescence technology: such as CEA, CA199, CA242, CA50, because of their low positive rate and lack of specificity (66.7%), it is not suitable for early diagnosis of colorectal cancer

[0007] (4) The use of PCR technology to detect DNA in serum or feces and to detect the concentration of microRNA has certain value in the monitoring of colorectal cancer treatment and prognosis evaluation, but their application in the early diagnosis of colorectal cancer still needs further development Research

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