Method for quickly inducing rice blast via box type seedling nursing

Abstract

The invention discloses a method for quickly inducing rice blast via box type seedling nursing. Paddy rice seedlings are cultured in sealed box type small environment; rice blast bacteria is inoculated during a fine rice-grain sprout stage and an inoculation morbidity result can be achieved in short period. The method comprises the following steps of (1) preparing matching nutrition solution, (2) filling seedling nursing soil, (3) broadcasting, (4) nursing paddy rice in the box type small environment, (5) preparing inoculant spore liquid, (6) inoculating bacteria and (7) managing nursing after inoculation. The method for quickly inducing rice blast via box type seedling nursing is advantageous in that the whole method requires less time and only eight days are required from broadcasting to typical morbidity; conditions having a close relationship with rice blast morbidity such as temperature, humidity and water and fertilizer are highly uniformed and can be easily controlled; the method can be simply operated, has a simple technology on the whole and can achieve technology standardization.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, it is a method for rapidly inducing rice blast by box-type seedling cultivation. Background technique [0002] Rice blast is a major disease of rice production in my country. In a pandemic year, it caused a loss of 15 billion kilograms of rice grain to my country. Although the current understanding of rice blast is quite in-depth, a large amount of research investment is still needed to achieve sustainable, safe and efficient control of rice blast. However, many studies on rice blast are inseparable from the basic method of artificially induced disease. Even the research in the molecular field, such as the problem of genes related to pathogenic bacteria or genes related to host disease resistance, often requires the results of artificially induced diseases to answer. . So far, the technology of artificially induced disease related to rice blas...

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Problems solved by technology

However, the currently widely used method of inducing disease at the seedling stage is to use the fully expanded seedling leaves to inoculate after the 3-5 leaf stage of the rice seedlings. It lasts more than 1 week, and the test cycle usually spans more than 3 weeks, which is suitable for some research work that requires short-term results, such as identification of pathogenic mutants in molecular plant disease research, expression of disease-resistant genes, and infection process It is still suspected that it takes too long to transmit information on the pathogen-host interaction; moreover, in order to obtain robust rice seedlings, sufficient sunlight and supplementary nutrients need to be provided, and the entire method system still needs to use a larger space such as a greenhouse ; The control of local conditions such as humidity, temperature, fertilizer and water during the test period also has great technical difficulties, or is quite limited by equipment conditions, etc.

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