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Colorectal cancer marker and application thereof

A marker, colorectal cancer technology, applied in the field of biomarkers, can solve the problem of lack of non-invasive early diagnosis methods for colorectal cancer, and achieve the effect of improving and restoring the intestinal microecological structure and preventing defects.

Active Publication Date: 2016-02-03
SHANGHAI REALBIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colon cancer has no obvious symptoms in the early stage, and there is currently a lack of effective non-invasive early diagnosis methods for colorectal cancer

Method used

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  • Colorectal cancer marker and application thereof
  • Colorectal cancer marker and application thereof
  • Colorectal cancer marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] In this example, the inventors carried out correlation analysis of intestinal flora microorganisms to study the characteristics of fecal microbial communities and functional components from fecal samples of 53 patients with colorectal cancer, 42 patients with colonic adenoma, and 61 healthy individuals. In general, a total of about 1084.87Gb of sequencing data of each sample in the above-mentioned control group was downloaded, and a colorectal cancer reference gene set was constructed. Quantitative metagenomic analysis revealed that 14,993 genes were significantly different (fdr<0.05) in a large number of patients and healthy controls. Most of the genes can be classified into 6 gene clusters (MetagenomicSpecies, MGS) representing bacterial species, of which 2 MGS were enriched in the colorectal cancer patient group, and 4 MGS were enriched in the healthy group.

[0066] 1. Download the sequencing data of the control group

[0067] The DNA sequencing data of stool sampl...

Embodiment 2

[0098] In order to verify that the species markers determined in Example 1 can be used as colorectal cancer markers, the MGS abundances of the remaining 47 healthy people and 31 colorectal cancer patients were used for verification. Wherein, the extraction and sequencing of DNA and the analysis of gene abundance were carried out with reference to the instructions of commercially available DNA extraction kits, the library construction and sequencing instructions of the selected sequencing platform, and the above steps.

[0099] The verification result is as image 3 shown. Taking the significance level α=0.05, the 4 markers determined in Example 1 that are significantly enriched in the healthy population have a significant difference in the abundance of the healthy group and the disease group in the verification group (P<0.05), as shown in Table 3. Eubacterium halllii is Enterobacter hallii, and it has been reported that enterobacterium hallii in the termite gut has a decompo...

Embodiment 3

[0104] Thirty-five stool samples were used to test the individual status of the sample source.

[0105]Determine the abundance of MGSH_1 and MGSH_2 in each stool sample with reference to the method in Example 2, that is, determine the abundance of Eubacterium_hallii_DSM_3353 and Clostridium in the sample, and compare whether the abundance of Eubacterium_hallii_DSM_3353 and Clostridium in each sample falls into the one determined in Example 2 The 95% confidence interval of MGSH_1 and MGSH_2 of the disease group or the healthy group, it is determined that the individual whose abundance of the two species falls into the corresponding interval of the disease group corresponds to a colorectal cancer patient, and it is determined that the abundance of the two species is equal The status of the individual corresponding to the sample falling into the corresponding interval of the healthy group is non-colorectal cancer patient.

[0106] The individual status of 31 of the samples can be...

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Abstract

The invention discloses a colorectal cancer marker, which consists of at least two of the following microorganisms: Eubacterium_hallii_DSM_3353, Clostridium_SS2_1, Ruminococcus_5_1_39BFAA and Eubacterium_ventriosum_ATCC_27560. The invention also discloses a method and an apparatus for determining individual status by virtue of the colorectal cancer marker. Compared with a colorectal cancer group, the colorectal cancer marker has significant enrichment in a health group; therefore, the colorectal cancer marker can serve as a marker for distinguishing the health group from the group of colorectal cancer patients.

Description

technical field [0001] The present invention relates to the field of biomarkers. Specifically, the present invention relates to colorectal cancer biomarkers and applications thereof. More specifically, the present invention relates to a colorectal cancer marker, the use of colorectal cancer markers, and a method for using colorectal cancer markers. A method for determining the state of an individual using a colorectal cancer marker, a device for determining the state of an individual using a colorectal cancer marker, a method for classifying a plurality of individuals using a colorectal cancer marker, a drug for treating colorectal cancer, and a method for preparing a treatment Approaches to colorectal cancer drugs. Background technique [0002] Colorectal cancer (Colorectal Cancer, CRC) is a cancer originating from the colon or rectum (a part of the large intestine). Due to abnormal growth of cells, it may invade or metastasize to other parts of the body. Patients with col...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/68C12M1/34C12R1/145C12R1/01
Inventor 仲文迪张林爽郑智俊
Owner SHANGHAI REALBIO TECH CO LTD
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