Ginkgo pollen fermented oral liquid and preparation method thereof
A technology for oral liquid and pollen, which is applied in the field of preparation of ginkgo pollen fermented oral liquid, can solve the problems of fermented odor and the development of health care functions, etc., and achieves the effects of good smell, enhanced immunity and strong immunity.
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Embodiment 1
[0027] (1) Use a vibration mill to pulverize the dried ginkgo pollen for 25 minutes to obtain broken ginkgo pollen;
[0028] (2) Mix broken ginkgo pollen, brown sugar, and purified water in a weight ratio of 5:7:88, and sterilize at high temperature to obtain a fermentation medium;
[0029] (3) The activated rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 liquid strains were respectively inserted into the MRS medium with an inoculum amount of 5%, and cultured at 37°C for 12 hours to obtain seed liquid; In this embodiment, the strain medium is not limited to the MRS medium, as long as the medium that can achieve the purpose of cultivating the two strains can be used in this embodiment;
[0030] (4) Add the seed solution of Rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 into the fermentation medium obtained in step b with an inoculum amount of 2% each, seal it with a plastic film, and put it under anaerobic conditions at 37°C. Set fermentation c...
Embodiment 2
[0033] (1) Use a vibration mill to pulverize the dried ginkgo pollen for 25 minutes to obtain broken ginkgo pollen;
[0034] (2) Mix broken ginkgo pollen, brown sugar, and purified water in a weight ratio of 6:8:86, and sterilize at high temperature to obtain a fermentation medium;
[0035] (3) The activated rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 liquid strains were respectively inserted into the MRS medium with an inoculation amount of 3%, and cultured at 37°C for 12 hours to obtain seed liquid;
[0036] (4) Add the seed solutions of Rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 to the fermentation medium obtained in step b with an inoculum amount of 4% each, seal the lid, seal it with a plastic film, and put it under anaerobic conditions at 37°C. Set fermentation culture, after measuring the pH value of the fermentation broth below 3.5, stop the fermentation;
[0037] (5) Filter the fermented liquid and add 8% honey to taste.
Embodiment 3
[0039] (1) Use a vibration mill to pulverize the dried ginkgo pollen for 25 minutes to obtain broken ginkgo pollen;
[0040] (2) Mix broken ginkgo pollen, brown sugar, and purified water in a weight ratio of 1:9:81, and sterilize at high temperature to obtain a fermentation medium;
[0041] (3) The activated rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 liquid strains were respectively inserted into the MRS medium with an inoculum amount of 2%, and cultured at 37°C for 12 hours to obtain seed liquid;
[0042] (4) Add the seed solution of Rhamnobacterium CGMCC 1.22 and Lactobacillus brevis CGMCC 1.558 to the fermentation medium obtained in step b with an inoculum amount of 0.5% each, seal it with a plastic film, and put it under anaerobic conditions at 37°C. Set fermentation culture, after measuring the pH value of the fermentation broth below 3.5, stop the fermentation;
[0043] (5) Filter the fermented liquid and add 8% honey to taste.
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