The preparation method of chitosan nanosphere
A technology of chitosan nano and chitosan, which is applied in the direction of nanotechnology, can solve problems such as complicated procedures, and achieve the effect of reducing costs and simple preparation methods
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Embodiment 1
[0031] (1) Dissolving 100mg chitosan in 40ml deionized water, stirring and mixing;
[0032] (2) add 2ml glacial acetic acid in the solution that step (1) obtains, stir until chitosan solid dissolves completely;
[0033] (3) Add 300 mg of EDTA disodium salt to the mixed solution obtained in step (2), stir and mix;
[0034] (4) Add dehydrated alcohol 14ml dropwise to the mixed solution obtained in step (3), until the solution becomes milky white;
[0035] (5) Transfer the mixed solution obtained in step (4) to a reaction kettle, the reaction temperature is 160°C, and the reaction is performed at a constant temperature for 10 hours;
[0036] (6) Cool the mixed solution obtained in step (5) to room temperature, centrifuge, wash with water, and dry at 60°C.
Embodiment 2
[0038] (1) Dissolving 100mg chitosan in 40ml deionized water, stirring and mixing;
[0039] (2) add 4ml glacial acetic acid in the solution that step (1) obtains, stir until chitosan solid dissolves completely;
[0040] (3) Add 300 mg of EDTA disodium salt to the mixed solution obtained in step (2), stir and mix;
[0041] (4) Add dehydrated alcohol 14ml dropwise to the mixed solution obtained in step (3), until the solution becomes milky white;
[0042] (5) Transfer the mixed solution obtained in step (4) to a reaction kettle, the reaction temperature is 160°C, and the reaction is performed at a constant temperature for 10 hours;
[0043] (6) Cool the mixed solution obtained in step (5) to room temperature, centrifuge, wash with water, and dry at 60°C.
Embodiment 3
[0045] (1) Dissolving 100mg chitosan in 40ml deionized water, stirring and mixing;
[0046] (2) add 6ml glacial acetic acid in the solution that step (1) obtains, stir until chitosan solid dissolves completely;
[0047] (3) Add 300 mg of EDTA disodium salt to the mixed solution obtained in step (2), stir and mix;
[0048] (4) Add dehydrated alcohol 14ml dropwise to the mixed solution obtained in step (3), until the solution becomes milky white;
[0049] (5) Transfer the mixed solution obtained in step (4) to a reaction kettle, the reaction temperature is 160°C, and the reaction is performed at a constant temperature for 10 hours;
[0050] (6) Cool the mixed solution obtained in step (5) to room temperature, centrifuge, wash with water, and dry at 60°C.
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