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Application of monoclonal antibody in treating psoriasis

A monoclonal antibody and psoriasis technology, applied in the direction of antibodies, medical preparations containing active ingredients, skin diseases, etc., can solve the problems of large side effects and easy recurrence

Active Publication Date: 2016-02-17
INNOVENT BIOLOGICS (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no effective treatment for psoriasis. Commonly used drugs include glucocorticoids, methotrexate, cyclosporine A, and biological therapy. Tolerance, etc.

Method used

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  • Application of monoclonal antibody in treating psoriasis
  • Application of monoclonal antibody in treating psoriasis
  • Application of monoclonal antibody in treating psoriasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Psoriasis therapeutic effect of bifunctional monoclonal antibody of the present invention

[0047] 1. Method

[0048] 1.1 Experimental objects and materials:

[0049] Balb / c mice, male, weighing 18-22 grams, Beijing Huafukang Biotechnology Co., Ltd., the quality certificate of experimental animals: SCXK (Beijing) 2009-0007.

[0050] Imiquimod ointment: Sichuan Mingxin Pharmaceutical Co., Ltd., content: 5%, batch number: 130901;

[0051] IBI-B: Innovent Biopharmaceutical (Suzhou) Co., Ltd., concentration: 40mg / ml, batch number: E20140402;

[0052] Methotrexate: Jiangsu Hengrui Pharmaceutical Co., Ltd., specification: 100mg / bottle, batch number: 20131103

[0053] 1.2 Grouping and administration

[0054] The mouse model of psoriasis was prepared by referring to the method of Leslie et al. [1]. According to the previous research results of our laboratory, Balb / c male mice were anesthetized by intraperitoneal injection of pentobarbital sodium (80mg / kg), and th...

Embodiment 2

[0124] Example 2 The inhibitory effect of the bifunctional monoclonal antibody of the present invention on the VEGF signaling pathway

[0125] 1. Experimental materials:

[0126] EndoGRO-VEGFCompleteCultureMediaKit: MilliporeSCME002

[0127] DMEM medium: Gibco11966-025

[0128] VEGF 165 : Peprotech100-20

[0129] CCK-8: Sigma96992

[0130] 2. Experimental steps:

[0131] 2.1 Digest and count HUVECs with good growth status.

[0132] 2.2 Resuspend endothelial cells with Millpore medium to 2X10 4 cells / ml, spread 2000 / well HUVECs (100ul) on a 96-well plate.

[0133] 2. After 324 hours, the medium was aspirated and replaced with DMEM dual medium containing 10ng / ml VEGF and 35nM IgG, VEGF-Trap, CR1, IBI-B, and a negative control without VEGF and a positive control containing VEGF but no antibody were set. A blank control with only PBS was also included. Three replicate wells were set up in each group for parallel experiments.

[0134] 2.4 After another 48 hours, add 10ul ...

Embodiment 3

[0138] Example 3 The inhibitory effect of the bifunctional monoclonal antibody of the present invention on the complement signaling pathway

[0139] 1.CH50 hemolysis test

[0140] 1.1 Experimental materials:

[0141] Sheep red blood cells: Guangzhou Ruite 007001

[0142] Hemolysin: Guangzhou Ruite

[0143] Human complement serum: SigmaS1764

[0144] Buffer GVB0: 0.1% gelatin, 5mM barbiturate, 145mM NaCl, adjust the pH to 7.2-7.4 and dilute to 1L

[0145] GVB++: 0.1% gelatin, 5mM barbiturate, 145mMNaCl, 0.15mM CaCL2, 0.5mMMgCL2, adjust the pH to 7.2-7.4 and then dilute to 1L

[0146] 0.1M sucrose GVB: Add 5.14g sucrose to 150ml GVB0

[0147] GVB-EDTA: 0.1% gelatin, 5mM barbiturate, 145mM NaCl, 10mM EDTA, adjust the pH to 7.2-7.4 and dilute to 1L

[0148] The above buffer nights were sterilized by high temperature and high pressure sterilizer before use

[0149] 1.2 Experimental steps:

[0150] 1.2.1 Sensitized sheep red blood cells

[0151] a. Wash sheep erythrocytes 3...

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Abstract

The invention discloses application of a dual-functional monoclonal antibody in treating psoriasis. Specifically, the invention provides application of the monoclonal antibody, and the monoclonal antibody is used for preparing a pharmaceutical composition for treating the psoriasis, wherein the sequence of the monoclonal antibody is as shown in SEQ ID NO.: 1. Experiments prove that the monoclonal antibody disclosed by the invention, by inhibiting a complement and by inhibiting VEGF (vascular endothelial growth factor) synergistically, achieves a therapeutic action on the psoriasis with an effect equal to that of a positive control group, and the action is significant in dose-dependent manner.

Description

technical field [0001] The term biomedical field of the present invention, specifically, relates to the application of a monoclonal antibody in psoriasis. Background technique [0002] Psoriasis, commonly known as psoriasis, is a chronic inflammatory skin disease with a long course of disease and a tendency to relapse, and some cases are almost unhealed throughout life. The disease mainly occurs in young and middle-aged people, and has a great impact on the physical health and mental status of patients. The clinical manifestations are mainly erythema and scales, and the disease can occur all over the body. The scalp and the extensor sides of the limbs are more common, and it usually aggravates in winter. The pathogenesis is complex, and it is currently believed to be related to genetics, infection, immune response, endocrine abnormalities, and mental factors. [0003] At present, there is no effective treatment for psoriasis. Commonly used drugs include glucocorticoids, me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K45/00A61P17/06
Inventor 李佳王春明俞德超
Owner INNOVENT BIOLOGICS (SUZHOU) CO LTD
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