Method for Doubling Chromosomes in Cotton Growth Point

Abstract

The invention relates to the technical field of agriculture plantation and concretely discloses a chromosome doubling method for cotton growing points. The method comprises following steps of: (1) asexual reproduction of plants; (2) preparation of growing points to be doubled; (3) drying treatment of growing points to be doubled; (4) pruning processing of growth points; (5) reversing of growing points; (6) chromosome doubling processing of growing points; (7) post processing and other steps. The chromosome doubling method for cotton growing points has following beneficial effects: due to the fact that distant hybridization F1-generation plants are subjected to grating of growth points, seedling strengthening, drying, pruning, reversing, soaking of sleeve pipes, doubling and other whole set of doubling technology, processed growth points are easily permeated by colchicines so that time and labor are saved; chromosome doubling efficiency of growth points of plants is increased so that medicine resources are effectively saved; the risky problem of seed soaking and doubling operation is solved by avoiding sterility of distant hybridization F1-generation plants and shortage of seeds; and fertility is achieved after chromosome doubling operation so that a basis is provided for growth cotton and other rare plants.

Description

technical field

[0001] The invention relates to the technical field of agricultural planting, in particular to a method for doubling chromosomes at growth points of plants. Background technique

[0002] Using wild cotton to innovate distant hybridization germplasm is an important way to solve the current limited genetic resources in cotton genetics and breeding. The vast majority of wild cotton are diploid cotton species (2n=2X=26), which originated in different regions of the world, and there is biological genetic isolation between them. On the one hand, distant hybridization is not easy to produce offspring; The F1 generation is highly sterile.

[0003] Especially for some rare distant hybrid F1 generations, because the number of seeds obtained is very small, the number of plants can only be increased by artificial sowing and grafting through asexual reproduction, and its sterility is often obtained by artificially doubling the chromosomes of the growth point of the plant...

Images