Photo-oxidation collagen crosslinking method and its application

A collagen cross-linking and photo-oxidation technology, which is applied in the field of medical supplies, can solve problems such as unstoppable disease, achieve low immunogenicity, improve biomechanical properties, and improve mechanical strength.

Active Publication Date: 2016-02-24
GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on the above, the photooxidative collagen cross-linking method is mainly used clinically as a therapy for treating diseases. It is relatively safe as a therapy, but due to the

Method used

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  • Photo-oxidation collagen crosslinking method and its application
  • Photo-oxidation collagen crosslinking method and its application
  • Photo-oxidation collagen crosslinking method and its application

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0038] Example 1

[0039] A method for crosslinking photooxidized collagen, which includes the following steps:

[0040] (1) Soak the decellularized scaffold in a photosensitizer solution with a concentration of 0.05 mg / ml for 30 minutes;

[0041] (2) Take out the soaked decellularized scaffold and place it in a petri dish, and irradiate it with 110nm wavelength ultraviolet light for 40 minutes; change the face when the ultraviolet light is irradiated for 20 minutes, and then continue to irradiate, the power density of the irradiated ultraviolet light is 3mw / cm 2 ;

[0042] (3) Soak in PBS solution for 0.6h, then shake for 0.6h;

[0043] (4) Change the PBS solution and soak for 0.6h, then shake for 0.6h.

Example Embodiment

[0044] Example 2

[0045] A method for crosslinking photooxidized collagen, which includes the following steps:

[0046] (1) Soak the decellularized scaffold in a photosensitizer solution with a concentration of 4.5 mg / ml for 55 minutes;

[0047] (2) Take out the soaked decellularized scaffold and place it in a petri dish, and irradiate it with 380nm wavelength ultraviolet light for 56 minutes; change the face after 28 minutes of irradiation, and then irradiate; in the step (2), the power density of irradiation is 5800mw / cm 2 ;

[0048] (3) Soak in PBS solution for 4h, then shake for 2h;

[0049] (4) Change the PBS solution and soak for 4 hours, then shake for 2 hours.

Example Embodiment

[0050] Example 3

[0051] A method for crosslinking photooxidized collagen, which includes the following steps:

[0052] (1) Soak the decellularized scaffold in 0.2mg / ml riboflavin sodium phosphate solution for 50 minutes;

[0053] (2) Take out the soaked decellularized scaffold and place it in a petri dish, and irradiate it with 365nm wavelength ultraviolet light for 30 minutes; the irradiation power density is 3000mw / cm 2 ; Change sides at the 15th minute, try to ensure even crosslinking on both sides.

[0054] (3) Soak in PBS solution for 3 hours, then shake for 1 hour;

[0055] (4) Change the PBS solution and soak for 3 hours, then shake for 1 hour.

[0056] In the foregoing embodiment, the decellularized scaffold may be a product after decellularization of animal tissues such as pigs, cattle, sheep, horses, rabbits, and monkeys. The photosensitizer can be a pigment photosensitizer such as NFK, 3-hydroxykynuric acid (3-OH-FK), Juncarin, riboflavin, FMN, FAD, retinal and the like. T...

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Abstract

The invention discloses a photo-oxidation collagen crosslinking method, comprising following steps: (1), soaking a decellularized scaffold in a riboflavin sodium phosphate solution 0.01-5 mg/ml for 1-60 min; (2), taking out the soaked decellularized scaffold, placing it in a culture vessel, exposing it to ultraviolet light 100-400 nm in wavelength for 1-60 min; (3), soaking the scaffold in a PBS solution for 0.5-4 h, and shaking for 0.5-2 h. The photo-oxidation collagen crosslinking method is used for preparing medical substitute materials. The method has the advantages that biomechanic characteristics such as mechanical strength of the decellularized scaffold can be effectively improved; as the biomechanic characteristics are mainly determined by collage fiber, collagen fiber bundles and the composition of their spatial structures, the biomechanic properties and stability of a decellularized material can be improved through photo-radiation/photosensitizer crosslinking.

Description

technical field [0001] The invention relates to the field of medical supplies, in particular to a method and application of photooxidized collagen cross-linking for treating decellularized tissues. Background technique [0002] The basic principle of photochemical collagen crosslinking is photooxidation reaction. According to the unique absorption spectrum of the photosensitizer, within its maximum absorption wavelength range, after being irradiated with a certain wavelength, the photosensitizer absorbs photon energy, is excited to form a triplet state, has a strong oxidation ability, and produces superoxide radicals or singlets. Oxygen, oxygen free radicals have the activity of directly modifying collagen molecules, causing the formation of covalent bonds in or between amino acid residues in collagen molecules, increasing the density of covalent bonds between collagen fibers, thereby improving the biomechanical properties of collagen (type II photochemical reaction). With...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24A61L27/50
Inventor 官习鹏易静楠
Owner GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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