Microwave extraction method for spirulina polysaccharide
A technology of spirulina polysaccharide and extraction method, which is applied in the field of polysaccharide preparation, can solve the problems of energy consumption, time consumption, and low yield, and achieve the effects of high extraction efficiency, reasonable process, and ideal yield
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[0017] Example 1
[0018] A microwave extraction method of spirulina polysaccharide, which passes through the following process steps:
[0019] (1) Microwave extraction: Weigh spirulina powder and add distilled water at a material-to-liquid ratio of 1:20, use an ultrasonic processor with a rated power of 150W, ultrasonic treatment time 3min, and then use intermittent microwave extraction for microwave extraction; the extract is fed In the centrifuge, the centrifuge speed is controlled to 5000r / min, the centrifugation time is 12min, and the supernatant is taken to obtain the spirulina polysaccharide crude extract; among them, the intermittent microwave extraction method is to control the microwave power of 500W, and the microwave radiation time of each cycle is 20S, intermittent Time 3min, repeat 6 cycles;
[0020] (2) Protein removal by TCA method: add an equal volume of 8% trichloroacetic acid solution to the crude spirulina polysaccharide extract obtained in step (1), shake for 1...
Example Embodiment
[0023] Example 2
[0024] A microwave extraction method of spirulina polysaccharide, which passes through the following process steps:
[0025] (1) Microwave extraction: Weigh spirulina powder and add distilled water at a material-to-liquid ratio of 1:10, ultrasonic processor rated power 100W, ultrasonic treatment time 5min, and then use intermittent microwave extraction method for microwave extraction; the extract is sent to the centrifuge In, control the centrifuge speed 4000r / min, centrifuge time 15min, take the supernatant to obtain the spirulina polysaccharide crude extract; among them, the intermittent microwave extraction method is to control the microwave power of 450W, the microwave radiation time of each cycle is 25S, and the intermittent time is 2min , Repeat 7 cycles;
[0026] (2) Protein removal by TCA method: add an equal volume of 5% trichloroacetic acid solution to the crude spirulina polysaccharide extract obtained in step (1), shake for 15 minutes; stand still for...
Example Embodiment
[0030] Example 3
[0031] A microwave extraction method of spirulina polysaccharide, which passes through the following process steps:
[0032] (1) Microwave extraction: Weigh spirulina powder and add distilled water at a material-to-liquid ratio of 1:30, ultrasonic processor rated power 200W, ultrasonic treatment time 2min; then use intermittent microwave extraction method for microwave extraction; the extract is sent to the centrifuge In, the centrifuge speed is controlled at 6000r / min, the centrifuge time is 10min, and the supernatant is taken to obtain the spirulina polysaccharide crude extract; among them, the intermittent microwave extraction method is to control the microwave power of 570W, the microwave radiation time of each cycle is 15S, and the intermittent time is 15min , Repeat 5 cycles;
[0033] (2) Protein removal by TCA method: add an equal volume of 10% trichloroacetic acid solution to the crude spirulina polysaccharide extract obtained in step (1), shake for 10 mi...
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