A kind of acidovorax nx-2 and its application in microbial degradation of cyclohexane
A technology of NX-2, acid-eating bacteria, applied in the field of cyclohexane degradation, to achieve the effects of stable operation, simple operation and low operation cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1: Isolation and Identification of Acidovorax NX-2
[0028] (1) Sample collection and domestication
[0029] Preliminary screening: Collect activated sludge from the sewage treatment tank of a pharmaceutical and chemical factory in Zhejiang on-site. After standing for 2 hours, the supernatant and suspended impurities are removed, leaving fine particles of sludge. The lower sludge after standing is mixed with Inorganic salt medium was mixed at 1:1 (v / v) as a mixed medium and put into the sludge acclimatization tank with a total volume of 5L, with cyclohexane as the substrate as the sole carbon source and energy source, (30±1°C ) constant temperature domestication culture, sludge is domesticated, during the domestication experiment, the domestication tank is stably aerated, and the pH value of the domestication tank is controlled to maintain at 7.0. When the substrate concentration drops below 50mg / L every day, add the substrate once to make After the addition, the...
Embodiment 2
[0038] Example 2 Preparation of Seed Liquid of Acidovorax NX-2 Strain
[0039] 1) Incline cultivation: Acidovorax NX-2 was inoculated on R 2 A solid slant culture medium, cultured at 30-32°C for 48-72 hours to obtain slant bacteria, the R 2 The final concentration of A solid slant medium is composed of: yeast powder 0.50g / L, casein 0.50g / L, soluble starch 0.50g / L, MgCl 2 ·7H 2 O 0.05g / L, tryptone 0.50g / L, glucose 0.50g / L, sodium pyruvate 0.30g / L, KH 2 PO 4 0.45g / L, agar 15~18g / L, pH 7.2, solvent is water.
[0040] 2) Seed culture: Use an inoculation loop to pick the slant bacteria obtained in step (1) and inoculate it into the seed medium for cultivation, add cyclohexane with a final concentration of 200 mg / L, and cultivate at 30-32°C for 48-72 hours to obtain OD 600 =0.3 bacterial liquid, the seed medium is an inorganic salt medium.
Embodiment 3
[0041] Embodiment 3: the performance of Acidovorax NX-2 (CCTCC NO: M 2015115) degrading cyclohexane
[0042] Using cyclohexane as the sole carbon source of Acidovorax NX-2, take the bacterium solution prepared by the method in Example 2 and inoculate it into fresh 50mL inorganic salt medium containing 93mg / L cyclohexane, so that the initial cell concentration (by OD) is 0.02, pH=7. Put it into a shaker with a temperature of 30°C and a rotation speed of 160r / min for cultivation, take samples every 3h to 4h to measure the degradation rate of cyclohexane, and use a 5mL syringe to draw out a part of the bacterial liquid to measure the OD value of the bacterial cells. The results are shown in image 3 . During the experiment, two parallel samples and a blank control group not inoculated with strains were designed. With the prolongation of time, the bacterial cell concentration gradually increased, and at 35 hours, the bacterial cell concentration reached a maximum of about 170 mg...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More