A kind of deodorization method of cod protein enzymatic hydrolyzate

A technology of protease hydrolyzate and cod, applied in the field of aquatic product processing, can solve the problems of reagent residue, low efficiency, yeast powder odor, etc., and achieve the effect of good application prospect and promotion value.

Active Publication Date: 2018-06-08
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The activated carbon adsorption method has a certain effect in deodorization and decolorization, but the loss of protein is relatively large; the macroporous resin adsorption method relies on the polarity of the macroporous resin and the properties of the surface groups to deodorize the sample, which is effective for the removal of fishy smell. Good effect and not easy to introduce impurities, but the treatment efficiency is low; acid-base treatment will introduce certain reagent residues; microbial fermentation methods such as yeast are only suitable for liquid samples, and the addition of yeast powder will introduce odor
[0004] At present, most of the deodorization methods used in actual production have certain defects, or introduce impurities, or have low efficiency. Therefore, the innovation or improvement of deodorization methods has become a research hotspot in the food field and an urgent problem to be solved

Method used

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  • A kind of deodorization method of cod protein enzymatic hydrolyzate
  • A kind of deodorization method of cod protein enzymatic hydrolyzate
  • A kind of deodorization method of cod protein enzymatic hydrolyzate

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Effect test

Embodiment 1

[0019] The deodorization treatment of cod protein enzymatic hydrolyzate by high-voltage pulsed electric field comprises the following steps:

[0020] Purchase fresh cod, slaughter it, remove the skin and viscera, take the back fish, and use a tissue grinder to pound it into minced meat. Configure a phosphate buffer solution with a pH of 8 (take 5.59g of dipotassium hydrogen phosphate and 0.41g of potassium dihydrogen phosphate, dissolve and set the volume to 1000ml, and then add cod minced meat to phosphate buffer with 20% substrate concentration In the solution, Bacillus subtilis alkaline protease was used as a tool enzyme, and the amount of enzyme added was 0.15%, added to the buffer solution, and stirred and enzymolyzed in a water bath at 55° C. for 4 hours. After the enzymolysis is completed, the enzymolysis solution is subjected to boiling water bath to inactivate the enzyme for 10 minutes. After the enzyme-inactivated enzymatic hydrolyzate was cooled, it was centrifuged...

Embodiment 2

[0034] For fresh cod, take the back fish and pound it into minced meat with a tissue grinder. Add cod meat mince at 20% as substrate concentration to phosphate buffer solution with pH=8, use Bacillus subtilisin alkaline protease as tool enzyme, add enzyme amount 0.15%, stir and enzymolyze in 55°C water bath for 4h. After the enzymolysis is completed, the enzymolysis solution is subjected to boiling water bath to inactivate the enzyme for 10 minutes. After cooling the enzymatic hydrolyzate after deactivation, it was centrifuged at 3500r / min for 10min, and the supernatant was taken for suction filtration.

[0035] Dilute cod protein enzymatic hydrolyzate by 15 times until the peptide content is about 2 mg / mL, the conductivity is less than 2000 μS / cm, and the viscosity is close to that of water, so that the diluted cod protein enzymatic hydrolyzate meets the requirements of high-voltage pulsed electric field for samples.

[0036] Before using high-voltage pulsed electric field e...

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Abstract

The invention provides a method of effectively removing fishy smell matters in Gadus protein hydrolysate. Fishy smell is removed by treating the Gadus protein hydrolysate with a high-voltage pulse electric field. The main fishy smell matters in the Gadus protein hydrolysate deodorized according to this technique are removed, a new idea is provided for aquatic deodorization, conditions are created for the development of functional foods made from aquatic protein hydrolysates, and the method has promising application prospect and good popularization value.

Description

technical field [0001] The invention belongs to the technical field of aquatic product processing, and in particular relates to a method for effectively removing main fishy substances in cod protein enzymatic hydrolyzate. Background technique [0002] Peptide fragments with physiological activity widely exist in cod protein enzymatic hydrolyzate, which play an important role in promoting growth, anti-oxidation, and lowering blood pressure. However, the ubiquitous fishy smell in aquatic products and the bad flavor produced in the enzymatic hydrolysis process seriously restrict the application of aquatic protein enzymatic hydrolyzate in the field of food resource development. Therefore, it is an urgent problem to effectively remove the fishy smell of cod protein enzymatic hydrolyzate. [0003] The deodorization methods of aquatic products are mainly divided into three categories: physical deodorization, chemical deodorization and biological deodorization. At present, the comm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/24
CPCC07K1/24C12P21/06
Inventor 李八方侯虎常钰菲张朝辉郑海旭
Owner OCEAN UNIV OF CHINA
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