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Method for selecting duodenal fluid sample for detecting pancreatic disease marker, and method for detecting pancreatic disease marker

A duodenal fluid and determination method technology, which is applied in the field of duodenal fluid samples, can solve problems such as small difference and poor detection accuracy, and achieve the effects of reducing missed detection, suppressing unnecessary inspection, and improving inspection performance

Inactive Publication Date: 2016-02-24
OLYMPUS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Actually, 30 minutes and 45 minutes after intravenous injection of pancreatic juice and bile secretion stimulators (secretin and CCK) were collected under fluoroscopy with a probe (Sonde) inserted into the duodenum The excreted pancreatic juice was measured for CEA (carcinoembryonicantigen, carcinoembryonic antigen) in the pancreatic juice obtained in this test (secretin test), so as to distinguish between healthy people, patients with chronic pancreatitis and patients with pancreatic cancer (refer to non- Patent Document 1) In this method, the difference in the CEA concentration region between the healthy group and the pancreatic cancer patient group is small, and it is expected that the detection accuracy will deteriorate if the number of cases increases

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  • Method for selecting duodenal fluid sample for detecting pancreatic disease marker, and method for detecting pancreatic disease marker
  • Method for selecting duodenal fluid sample for detecting pancreatic disease marker, and method for detecting pancreatic disease marker
  • Method for selecting duodenal fluid sample for detecting pancreatic disease marker, and method for detecting pancreatic disease marker

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Embodiment

[0113] Next, the present invention will be described in more detail by showing examples and the like, but the present invention is not limited to the following examples.

reference example 1

[0115] Duodenal fluid samples were collected endoscopically from 49 pancreatic cancer cases, and the CEA concentration in the samples was measured. The CEA concentration was measured by the ELISA method using a commercially available kit (manufactured by IBL). Based on the measured CEA concentration, the critical value was set at 126ng / mL to judge negative and positive.

[0116] The sensitivity and the ratio of the number of positive samples to the total number of samples obtained from the test results are shown in Table 1 for each T classification indicating the degree of local development of the pancreas of the main lesion. Tis refers to non-invasive cancer; T1 refers to tumor diameter less than 2 cm and confined to the pancreas; T2 refers to tumor diameter exceeding 2 cm and confined to the pancreas; T3 refers to cancer invasion involving the bile duct in the pancreas, duodenum, and surrounding tissues of the pancreas Any one; T4 refers to any one in which the cancer infil...

Embodiment 1

[0121] Absorption spectra were measured for duodenal fluid samples collected endoscopically from 49 cases of pancreatic cancer and 7 cases of benign pancreatic diseases. The absorption spectra of all samples to be tested are shown in Figure 4 . Will Figure 4 The magnified diagram near the absorption peak (300-560nm) in Figure 5 . It can be seen that the duodenal fluid sample has an absorption peak wavelength region at 350 to 540 nm, especially at 400 to 460 nm.

[0122] Furthermore, the density of the color of each duodenal fluid sample (specimen) was evaluated. Specifically, using a spectrophotometer, the absorption spectrum (A 10 ). Next, based on the following formula, the obtained absorption spectrum was converted into a transmission spectrum (T 10 ), and further take the optical path length 5mm (T 5 ) for conversion.

[0123] T 10 = 10 - A ...

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Abstract

Provided are: a method whereby, before detecting a pancreatic disease marker, the qualities of duodenal fluid samples are evaluated so as to exclusively select a sample to be subjected to the pancreatic disease marker detection, said sample having a high possibility of containing pancreatic juice and therefore being highly suitable as a sample therefor; and a method for detecting a pancreatic disease marker by using the duodenal fluid sample that is selected by the aforesaid method. The method for selecting a duodenal fluid sample for detecting a pancreatic disease marker is characterized by comprising: (a1) a step for comparing the color depth of duodenal fluid samples with a definite standard color; and (b1) a step for determining which duodenal fluid sample is to be subjected to a pancreatic disease marker test, said sample having a color depth being the same as or higher than the standard color, and which duodenal fluid sample is not to be subjected to the pancreatic disease marker test, said sample having a color depth being lower than the standard color.

Description

technical field [0001] The present invention relates to determining the suitability of a sample that can give a highly reliable result when a duodenal fluid sample is tested for the detection of a pancreatic disease marker, thereby determining ten samples suitable for the detection of a pancreatic disease marker. A method for a duodenal fluid sample; and a method for detecting pancreatic disease markers using the duodenal fluid sample determined by the method. [0002] This application claims priority based on Japanese Patent Application No. 2013-138848 for which it applied in Japan on July 2, 2013, and uses the content here. Background technique [0003] Pancreatic juice (body fluid discharged from the pancreatic duct) is an important biological sample for knowing the state of the pancreas, and is used in cell diagnosis, bicarbonate measurement, bacterial inspection, and inspection of markers made of proteins and nucleic acids, etc. Pancreatic disease examination. In part...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01J3/42G01J3/46G01N33/50G01N33/52G01N33/68
CPCG01N33/48G01N2800/067G01N21/293A61B2010/0061A61B5/1032A61B5/4283G01N33/728G01N21/29G01N21/31G01N33/57438G01N33/6872G01N2333/70503
Inventor 武市理惠守屋奈绪佐贯博美中田真理清原正伸武山哲英长冈智纪
Owner OLYMPUS CORP