Method for preparing giant phospholipid vesicle array through point-surface electrode field on basis of contact print technology
A technology of micro-contact printing and giant phospholipids, which is applied in the preparation of test samples, etc., can solve the problem of single-size control of giant phospholipid vesicles, and achieve good monodispersity of vesicles, single and controllable size, and wide distribution range Effect
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[0025] Specific embodiment 1: This embodiment is a method for preparing a giant phospholipid vesicle array based on micro-contact printing technology using a point surface electrode electric field, which is completed in the following steps:
[0026] 1. Cleaning the electrode: first use anhydrous ethanol to ultrasonically clean the ITO electrode, then use distilled water to ultrasonically clean the ITO electrode, then use nitrogen to dry, and then use a plasma cleaner to clean the ITO electrode; get the cleaned ITO Electrode; soak the tungsten needle electrode in hydrochloric acid with a substance concentration of 2mol / L for 3h-6h, then rinse it with distilled water 3-6 times, and then dry it with nitrogen to obtain a cleaned tungsten needle electrode;
[0027] 2. Cleaning of patterned polydimethylsiloxane seal: first use distilled water to ultrasonically clean the patterned polydimethylsiloxane seal, and then use absolute ethanol to clean the patterned polydimethylsiloxane seal. Th...
Example Embodiment
[0043] The second embodiment: The difference between this embodiment and the first embodiment is: the number of times of ultrasonic cleaning of the ITO electrode with absolute ethanol in step one is 2 to 3 times, and the time of each ultrasonic cleaning is 5min~20min, the ultrasonic power is 40W~80W. Others are the same as the first embodiment.
Example Embodiment
[0044] Specific embodiment three: The difference between this embodiment and specific embodiment one or two is: the number of ultrasonic cleaning of ITO electrode with distilled water in step one is 2 to 3 times, and the time of each ultrasonic cleaning is 5min~20min, the ultrasonic power is 40W~80W. Others are the same as the first or second embodiment.
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