A method of purifying sodium chondroitin sulfate

A technology of sodium chondroitin sulfate and ethanol precipitation, applied in the field of purifying sodium chondroitin sulfate, can solve the problem of high cost, achieve the effects of small environmental pollution, low cost, and improve product quality and appearance

Inactive Publication Date: 2016-03-16
QINGDAO JIULONG BIO PHARMA
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  • Abstract
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Problems solved by technology

Some methods are costly, and some methods are d

Method used

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Embodiment Construction

[0012] (1) Reduction: Adjust pH 5.0-7.0 to 500L of the dissociated solution after alkaline hydrolysis and enzymolysis, then raise the temperature to 55-70°C, add 4kg of sodium pyrosulfate, stir for 25-35 minutes, and let stand for 25 - After 35 minutes, filter;

[0013] (2) Ethanol precipitation: cool the filtrate obtained in step (1) to below 30°C, keep the pH at 5.0-7.0, add ethanol while stirring, so that the ethanol concentration is 55-70 degrees, and let it stand for 6-8 hours. The precipitate was separated by centrifugation.

[0014] (3) Ethanol re-precipitation: dissolve the precipitate in step (2) with 670L of purified water, add 7kg of sodium chloride, add ethanol while stirring, so that the degree of ethanol precipitation reaches 55-70 degrees, and let it stand for 6-8 hours.

[0015] (4) Sterilization: suck out the supernatant in step (3), add ethanol to the precipitate while stirring, make the ethanol precipitation reading reach 80-85 degrees, and let stand for 10...

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Abstract

The invention relates to a method of purifying sodium chondroitin sulfate. The sodium chondroitin sulfate is a mucopolysaccharide compound prepared by extracting pig nasal bone and other animal cartilage. A product of the sodium chondroitin sulfate, the content of which is higher than 95%, is prepared by subjecting the animal cartilage to alkaline hydrolysis-enzymolysis extraction, adding a reductant, reducing to obtain a decomposition solution, and performing two times of ethanol precipitation. The method is simple in process, mild in conditions and safe in production, and the cost of the method is lower than costs of adsorption, column chromatography and other methods. The method is suitable for large-scale industrial production.

Description

technical field [0001] The field of the invention belongs to the field of biopharmaceuticals, and relates to a method for purifying sodium chondroitin sulfate. Background technique [0002] Sodium chondroitin sulfate is an acidic mucopolysaccharide extracted from animal cartilage tissue, which belongs to glycosaminoglycan. Sodium chondroitin sulfate is mainly used in the treatment of rheumatism and rheumatoid diseases. It also has anticoagulant, anticancer, antithrombotic, and blood lipid-lowering effects. It can be used to treat symptoms such as headache, migraine, coronary heart disease, and angina. [0003] At present, domestic techniques for separation and purification of sodium chondroitin sulfate include ethanol precipitation, ion exchange chromatography, cellulose separation, and adsorption. Some methods are costly, and some methods are difficult to apply in industrial mass production. The ethanol precipitation method has simple process, mild conditions, safe produc...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069
Inventor 刘冠男殷文杰迟培升
Owner QINGDAO JIULONG BIO PHARMA
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