Enoxaparin sodium purifying method
The technology of enoxaparin sodium and purification method is applied in the field of preparation of biological pharmaceutical raw materials, which can solve the problems of inability to achieve clarity and poor clarity, and achieve the effects of good clarity and simple purification operation.
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[0018] Put 1.1 kg of the prepared crude enoxaparin sodium into 5 L of 10% sodium chloride solution, stir and dissolve to obtain a crude enoxaparin sodium solution, and filter to obtain a filtrate. Put the filtrate in a centrifuge at 3000r / min for 30min to obtain supernatant and precipitate. 10L of ethanol was added to the supernatant, stirred for 1 h and then allowed to stand for 3 h to obtain a precipitate. The precipitate was dissolved into a 10wt~15wt% solution with purified water, filtered through a 0.22μm filter membrane, and then spray-dried to obtain 1.02kg of enoxaparin sodium finished product, with a purification yield of 93.5%.
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