High-throughput seed automatic sampling and positive seed selection system and method

Abstract

The invention relates to an automated high-throughput seed sampling and positive seed selecting system and method. The system is characterized in that an operating platform and an on-line computer are included, and the surface of the operating platform is provided with a seed arranging area, a sampling area and a preserving area; the surface of the seed arranging area is provided with a seed vibration plate and a seed conveyor belt which are used for arranging seeds, a seed box conveying rail is arranged at the position adjacent to the seed vibration plate, and a first board position is arranged on the seed box conveying rail; a sampling box conveying rail is arranged in a groove formed in the surface of the sampling area, and a second board position is arranged on the sampling box conveying rail; one end of the sampling box conveying rail is provided with a laser gun moving rail and a laser gun which are used for cutting seed endosperms; a first guide rod for transferring seed boxes to the sampling area from the seed arranging area is arranged over the sampling box conveying rail; a third board position and a fourth board position are arranged on the surface of the preserving area side by side; a second guide rod for transferring the seed boxes to the preserving area from the sampling area is arranged over the preserving area. The system and method can be widely applied to the field of automated seed sampling and positive seed selecting.

Description

Technical field [0001] The invention relates to the technical field of molecular breeding, in particular to a high-throughput seed automatic sampling and positive seed selection system and method. Background technique [0002] Biotechnology and breeding research, as well as the seed industry are facing severe challenges and crises. The transformation from traditional conventional breeding technology to relying on biotechnology, information technology, and automation is an inevitable choice for the development of modern agriculture. The basic prerequisite for achieving high-throughput molecular breeding is to first achieve high-throughput extraction of different high-purity DNA / RNA / protein / metabolites. This is currently one of the rate-limiting steps restricting the efficiency of biotechnology and molecular breeding. [0003] The conventional analysis method is to first plant a large number of populations in the field, and extract the DNA / RNA from the leaves of the plants. The use ...

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Problems solved by technology

5) Purification of inbred lines in conventional breeding. For example, some inbred lines still segregate in individual traits after 7 generations. Homozygous individuals are easily selected for planting in the field

At present, domestic conventional seed sampling is to manually use a small drill to drill the endosperm, which has many problems such as low work efficiency, difficulty in obtaining a useful sample size, serious pollution risks, low sampling accuracy affecting the germination rate, and heavy dependence on the skills of the staff.

For example, the corn seed cutter (application number: CN201220278079, publication number: CN202622864U) invented by Zhang Chunqing of Shandong Agricultural University and others can cut a row of seeds at a time by using a guillotine, but in order to avoid pollution, it is necessary to cut the cutter after cutting a row of seeds. Questions about cleaning or replacing

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