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Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof

A technology for immunochromatographic test strips and Mycoplasma pneumoniae, which is applied to measurement devices, analytical materials, instruments, etc., can solve the problems that the test results cannot meet the needs of clinical diagnosis, cannot achieve rapid and accurate detection, and are inaccurate, and achieve the realization of The effect of early diagnosis of infection, short detection time and strong specificity

Inactive Publication Date: 2016-03-23
姜竹泉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ELISA detection method and immunochromatography method are widely used to detect IgG or IgM. Since the human body needs certain conditions and time to produce antibodies, they all have certain hysteresis and inaccuracy, and cannot achieve the purpose of rapid and accurate detection.
The test results cannot meet the needs of clinical diagnosis

Method used

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  • Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof
  • Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof
  • Collaurum immunochromatography test strip detecting mycoplasma pneumoniae and preparing method thereof

Examples

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Effect test

Embodiment 1

[0048] Example 1 Such as figure 1 As shown, the present invention discloses a colloidal gold immunochromatographic test strip for detecting Mycoplasma pneumoniae, comprising a sample pad, a binding pad (gold pad), a nitrocellulose coating, a water-absorbing pad and a PVC bottom plate, the sample pad, The binding pad, the nitrocellulose coating film and the water-absorbing pad are sequentially lapped on the PVC bottom plate: the binding pad is coated with MP-h1 antibody-colloidal gold-carbon nanotube markers; A detection line and a quality control line are provided on the nitrocellulose coating membrane, the detection line is coated with MP-h1 antibody, and the quality control line is coated with goat anti-mouse IgG.

Embodiment 2

[0049] Embodiment 2 A kind of preparation method of the colloidal gold immunochromatography test strip that detects Mycoplasma pneumoniae

[0050] Include the following steps:

[0051] Step 1. Take a 250mL beaker, add 99mL of pure water to a 250mL Erlenmeyer flask, and heat it on a constant temperature magnetic stirrer with a temperature range of 200°C at a speed of 200r / min. The actual temperature was 100°C, and 1 mL of 1% trisodium citrate solution was quickly added to the beaker. Quickly press the timer, and quickly add 1mL of 1% chloroauric acid solution after 1 minute. The solution in the beaker turned red and then heated for 10 minutes until the solution became clear, cooled to room temperature, and fixed to volume in a 100 volumetric flask, and measured whether the maximum absorption wavelength was 525nm by an ultraviolet spectrophotometer as a test standard. That is, the colloidal gold was prepared at 4°C, and sealed and stored;

[0052] Step 2, preparation and pu...

Embodiment 3

[0054] The pretreatment of embodiment 3 sample pad and gold standard pad

[0055] Including the step of pre-treating the sample pad and the gold standard pad, that is, cutting the sample pad and the gold standard pad into strips with a width of 4 mm with a strip cutter, and using 5% sucrose, 5% BSA and a final volume concentration of Soak in 0.1% 0.01mol / L PBS solution with a pH of 7.4, drain the solution after soaking, and dry at 37°C for 12 hours for later use.

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Abstract

The invention belongs to a collaurum immunochromatography test strip detecting mycoplasma pneumoniae, comprising a sample pad, a combining pad, a nitrocellulose coating film, a water absorption pad and a PVC bottom plate; the sample pad, the combining pad, the nitrocellulose coating film and the water absorption pad are overlapped on the PVC bottom plate in sequence; the combining pad is coated with a mycoplasma pneumoniae antibody MPh2-collaurum-carbon nanotube marker; the nitrocellulose coating film is provided with a detection line and a quality control line; the detection line is coated with a mycoplasma pneumoniae antibody MPh1 and the quality control line is coated with a goat anti mouse IgG. The invention further comprises a preparing method of the collaurum immunochromatography test strip, comprising the steps of preparing of collaurum, preparing of a gold marker antibody, purifying and assembling of the test strip. The collaurum immunochromatography test strip has the characteristics of high specificity, high sensitivity, simple and convenient operation, fast detection, accuracy and suitability for field use.

Description

technical field [0001] The invention relates to the technical field of pathogen detection in patients with mycoplasma pneumoniae infection, in particular to a colloidal gold immunochromatographic test strip for detecting mycoplasma pneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae, MP) is a common pathogen of human respiratory tract infection of all ages worldwide. It is the only mycoplasma pathogen that can cause respiratory tract infection, causing upper respiratory tract infection, tracheitis, bronchitis, bronchiolitis, community-acquired pneumonia, and can aggravate bronchial asthma. MP can also cause many extrapulmonary manifestations and systemic diseases, such as encephalitis, Guillain-Barré syndrome, pericarditis, arthritis, autoimmune anemia, renal dysfunction, asthma and Crohn's disease. Quickly and accurately judging whether a patient is infected with Mycoplasma pneumoniae is of great significa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/531
CPCG01N33/56933G01N33/531G01N33/558
Inventor 徐慰倬宋明姜竹泉
Owner 姜竹泉
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