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A kind of individualized drug detection kit for receptor blockers

A detection kit and kit technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of high false positive rate, high cost, long cycle, etc., and achieve simple identification , fast response and simple steps

Active Publication Date: 2019-01-11
BEIJING JINQI BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection products of ADRB1 gene polymorphism mainly use traditional solid-phase chips, liquid-phase chips and fluorescent quantitative PCR, etc. These technical means have defects such as high cost, long cycle time and high false positive rate to varying degrees.

Method used

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  • A kind of individualized drug detection kit for receptor blockers
  • A kind of individualized drug detection kit for receptor blockers
  • A kind of individualized drug detection kit for receptor blockers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0055] Extract 1ml of venous blood from 4 people and store it in EDTA anticoagulant tubes. Take 200ul of blood from each tube, use DNA extraction kits from Quanshijin or other companies, refer to the kit instructions to extract whole genome DNA as a template for detection, and mark them as M1, M2, M3 and M4. The detection reaction is carried out with the kit of the present invention, and the implementation effect of the kit of the present invention is verified.

[0056] Using Allele-Specific LAMP technology for template M, the nucleic acid to be detected is subjected to LAMP reaction, which is divided into two groups of reaction systems, one group is system C for C alleles, and the other is system G for G alleles. Template Mn (n is the group number, 1-4) was added to system C and system G respectively to obtain reaction systems C and G.

[0057] First, select the primer set as described in the first aspect of the application to react the sequence of the sample, and the primer...

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Abstract

The invention belongs to the technical field of biological detection, and in particular relates to an individualized medication detection kit for a receptor blocker. A detection primer group comprises a positive direction outer side primer F3: SEQ ID NO:1, a negative position outer side primer B3: SEQ ID NO:2, a positive direction inner side primer FIP: SEQ ID NO:3, a negative direction inner side primer BIPC: SEQ ID NO:4 and a negative direction inner side primer BIPG: SEQ ID NO:5. The detection kit provided by the invention is simple in step, quick in response, efficient, high in specificity and simple and convenient in identification, so that the detection kit has a broader application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to an individualized drug detection kit for receptor blockers, in particular to a detection kit for ADRB1 gene polymorphism and a reaction system composed thereof. Background technique [0002] β-adrenergic receptor (β-adrenergic receptor) is a subfamily of adrenergic receptors, belonging to the G protein-coupled receptor superfamily, including β1, β2 and β3 three different subtypes. This type of receptor regulates intracellular cAMP and L-type Ca by coupling with Gs protein 2+ The opening frequency of the channel is the target of β-agonists and β-blockers. The emergence of ADRB1Gly389Arg (rs1801253) polymorphic site will lead to the production of two types of receptors, Arg389 and Gly389, and researchers have confirmed that the coupling efficiency of Arg389 receptors to G proteins is significantly higher than that of Gly389 receptors. Therefore, if hypertensive patient...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6883
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD